冬眠及外源维生素C对中华鳖(Pelodiscus sinensis)稚鳖能量代谢的影响  

EFFECT OF DIETARY VITAMIN C AND HIBERNATION ON ENERGY METABOLISM IN JUVENILE SOFT-SHELLED TURTLE(PELODISCUS SINENSIS)

作　　者：井润贞[1,2] 牛翠娟[1] 钱美亚[1] 黄晨西[1] 张左兵[1] 钱亚群[1] 

机构地区：[1]北京师范大学生命科学学院,生物多样性与生态工程教育部重点实验室,北京100875 [2]天津农学院水产科学系,天津市水产生态及养殖重点实验室,天津300384

出　　处：《北京师范大学学报（自然科学版）》2011年第2期185-191,共7页Journal of Beijing Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(30671598;30271014);天津农学院青年科技发展基金资助项目

摘　　要：观测了冬眠前饲料中添加外源维生素C(Vc)对冬眠中华鳖稚鳖能量代谢的影响.结果发现外源Vc不能提高冬眠期间中华鳖的存活率(P=0.57).稚鳖个体在冬眠期间生长能的平均值为-0.15 kJ.d-1,该值与代谢耗能相当.鳖体蛋白、脂肪、水分和灰分质量分数w在冬眠前后无显著差异(P>0.05),外源Vc对鳖的体组成没有明显影响(P>0.05).冬眠8周后,肝脏线粒体细胞色素C氧化酶(COX)和肝脏乳酸锐氢酶(LDH)的活性显著下降(P<0.05).饲料外源Vc对肝脏线粒体蛋白含量、肝脏线粒体COX和LDH的活性均无影响(P>0.05).The effect of dietary vitamin C （Vc） on energy metabolism in juvenile soft-shelled turtle Pelodiscus sinensis during hibernation was measured. Turtles （29.41±5.44 g） in all 3 groups were fed dietary vitamin C at 0 mg.kg-1 （Vc0）, 500 mg.kg-1 （Ve500） and 2 500 mg-kg 1 （Vc2500） for 4 weeks at 25℃, entered hibernation at 7℃ and then hibernated for 8 weeks. Another group was fed the 0 mg-kg-1 Vc diet 25℃ throughout the experiment （Vc0C）. Data revealed that dietary Vc had no effect on turtle survival rate during hibernation （P〉0. 05）. The average growth energy was --0. 15 kJ·ind-1·d -1, equal to metabolic energy consumed during hibernation. The body chemical composition of the turtles changed little during hibernation （P〉0.05）. Dietary Vc showed no significant effect on body composition （P〉0.05）. Liver eytochrome c oxidase and lactate dehydrogenase activities decreased after hibernation （P〈.0 05）. Liver mass-specific protein content, cytochrome c oxidase and lactate dehydrogenase activities were not significantly affected by dietary Vc （P〉0. 05）.

关 键 词：中华鳖 维生素C 冬眠 能量代谢 体组成 细胞色素C氧化酶 乳酸脱氢酶 

分 类 号：S966.5[农业科学—水产养殖]