中国北方汉族人群TRIB1基因rs17321515多态性与2型糖尿病的相关性研究  

Relationship between TRIB1 Gene rs17321515 Polymorphisms and Type 2 Diabetes Mellitus in Han Nationality from Northern China

作　　者：李琳[1] 鄢盛恺[2] 夏良裕[1] 杨辉[2] 金勋[2] 李爽[2] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院检验科,北京100730 [2]卫生部中日友好医院检验科,北京100029

出　　处：《现代检验医学杂志》2011年第2期7-11,共5页Journal of Modern Laboratory Medicine

摘　　要：目的 探讨中国北方汉族人群TRIB1基因rs17321515多态性与2型糖尿病的关系.方法 应用聚合酶链反应限制性片段长度多态性（PCR-RFLP）技术检测了148例对照组,98例2型糖尿病组和76例2型糖尿病并发冠心病组TRIB1基因rs17321515多态性基因型,分析了不同组间基因型和等位基因频率分布特点,并探讨了基因多态性对糖化血红蛋白（HbA1c）、血糖、血脂水平的影响.结果 三组研究对象间的TRIB1基因rs17321515多态性基因型和等位基因频率分布差异无统计学意义（P〉0.05）.中国北方汉族人群rs17321515多态性基因型AA,AG,GG频率分别为0.151,0.500,0.349,A,G等位基因频率分别为0.401,0.599,与不同国家地区间人群分布差异无统计学意义（χ^2=3.543,P=0.471）.2型糖尿病并发冠心病组中AG＋GG基因型患者TG水平明显高于AA型,差异有统计学意义（P〈0.05）.未发现TRIB1基因rs17321515多态性与性别相关.Logistic回归分析显示,A等位基因是2型糖尿病并发冠心病的危险因素（95%CI=1.032～4.742,OR=2.212,P=0.041）.结论 TRIB1基因rs17321515多态性与2型糖尿病并发冠心病有关联,A等位基因可能是我国北方地区汉族人群2型糖尿病并发冠心病发病的危险因素.Objective To explore the interaction between TRIB1 gene rs17321515 single-nucleotide polymorphism （SNP） and some classic risk factors in patients with type 2 diabetes mellitus （T2DM） in Han nationality from Northern China. Methods Genotypes of TRIB1 rs17321515 polymorphisms were analyzed by using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） in 148 unrelated healthy individuals, 98 patients of T2DM,and 76 patients of T2DM combined coronary heart disease （CHD）. The association of TRIB1 gene rs17321515 SNP with levels of hemoglobin Alc（HbAlc） ,serum lipids,and glucose was also assessed. Results Neither the frequencies of genotypes nor frequencies of alleles of rs17321515 polymorphisms were stetistically different among DM patients ,DM ＋CHD patients and controls （P〉 0.05）. The genotype frequencies of AA, AG ,GG were 0. 151,0. 500,0. 349, respectively;the allele frequencies of A and G were 0. 401,0. 599 ,respectively. There was no significant difference in the frequencies of genotypes or alleles of rs17321515 polymorphisms between Han nationality from Northern China and other populations abroad （χ^2= 3. 543,P= 0. 471）. In the DM＋CHD group,triglycerides （TG） levels of G allele gene carriers were statistically higher than that of AA genotype （P 〈0. 05）. No relationship between gender and TRIB1 rs17321515 polymorphisms was found. Logistic regression analysis showed that A allele was the risk factor for T2DM with CHD （95%CI=1. 032;4. 742,OR=2. 212,P=0. 041）. Conclu- sion The rs17321515 polymorphisms of TRIB1 gene showed no significant correlation with T2DM,but showed the association with T2DM combined CHD. A allele of TRIB1 gene rs17321515 may be the genetic risk factor of T2DM with CHD in Han nationality from Northern China.

关 键 词：TRIB1 基因多态性 糖尿病 冠心病 聚合酶链反应限制性片段长度多态性 

分 类 号：Q781[生物学—分子生物学] R587.1[医药卫生—内分泌]