柑桔绿霉病拮抗细菌的筛选、鉴定及其抑制效果  

Selection of Antagonistic Bacteria against Citrus Green Mold and Its Inhibition Activity on Shatang Mandarin Fruit

作　　者：郝卫宁[1] 李辉[1] 胡美英[1] 耿鹏[1] 杨柳[1] 

机构地区：[1]华南农业大学天然农药与化学生物学教育部重点实验室,广州510642

出　　处：《中国生物防治学报》2011年第2期284-288,共5页Chinese Journal of Biological Control

基　　金：国家自然科学基金(30871660);广东省科技计划项目(2008B050100045)

摘　　要：从采集的柑桔园根际土壤中筛选得到1株对柑桔绿霉菌Penicillium digitatum具有较强拮抗活性的菌株HC-03。该菌株对柑桔绿霉菌、柑桔青霉菌Penicillium italicum、柑桔酸腐菌Geotrichum candidum等12种水果病原菌均有不同程度的拮抗作用,表现出广谱抗菌活性。菌落形态观察、生理生化特性分析及16S rDNA序列测定和特异性PCR检测结果表明,该菌株为解淀粉芽孢杆菌Bacillus amyloliquefaciens。对沙糖桔的活体接种试验表明,菌株HC-03的发酵液、菌悬液及发酵滤液处理柑桔绿霉病的发生率均低于20%,病斑直径均在10mm以下。A bacterial strain HC-03,isolated from rhizosphere soil of citrus farms,exhibited extensive antagonistic effect on 12 fruit pathogens.The strain was identified as Bacillus amyloliquefaciens based on 16S rDNA sequence homology,specific PCR detection,physiological and biochemical characteristics and morphological observation.Effect of different treatments of strain HC-03 against disease caused by Penicillium digitatum on inoculated shatang mandarin was also investigated.Results showed that citrus green mold incidence of shatang mandarin treated by cell culture,cell-free filtrate and cell suspension of strain HC-03 were all less than 20%,and lesion diameter were all below 10 mm.

关 键 词：柑桔绿霉菌 拮抗细菌 解淀粉芽孢杆菌 沙糖桔 抑制作用 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S436.661.1[农业科学—植物保护]