Spatio-Temporal Expression Patterns of Aurora Kinases A, B in Mouse Zygotes during the First Mitosis  

作　　者：Jie ZHANG Lin XU Guo-li WANG Divya RANA Tong LIU Di-di WU Bing-zhi YU 

机构地区：[1]Department of Biochemistry and Molecular Biology, China Medical University, Shenyang 110001, China [2]Department of Gynecology and Obstetrics, Shengjing Hospital of China Medical University, Shenyang110003, China

出　　处：《Journal of Reproduction and Contraception》2011年第1期11-21,共11页生殖与避孕（英文版）

基　　金：supported by funds from the National Natural Science Foundation (81070527) of China

摘　　要：Objective To investigate the expression and localization of Aurora kinase A （A URKA ） and Aurora kinase B （A URKB） in mouse zygotes during the process of the first mitosis. Methods Quantitative real-time RT-PCR and Western blotting were performed to analyze the expression of AURKA and AURKB. The subcellular location of AURKA and A URKB was studied by confocal microscopy. Results A URKA and A URKB were increasingly expressed from phase G1 and peaked at phase M. After the entrance into mitosis A URKB became the predominant form both in mRNA and protein levels. The proteins of A URKA and A URKB both distributed in the cytoplasm and were associated with nucleus during the first mitosis of mouse zygotes, with some details in different. Conclusion The expression and localization of Aurora kinases A and B was in a cell- cycle regulated manner during the process of the cleavage of mouse zygotes. This discovery will aid in future investigations on their specific roles and molecular mecha- nisms in the regulation of mammalian early embryonic development.Objective To investigate the expression and localization of Aurora kinase A （A URKA ） and Aurora kinase B （A URKB） in mouse zygotes during the process of the first mitosis. Methods Quantitative real-time RT-PCR and Western blotting were performed to analyze the expression of AURKA and AURKB. The subcellular location of AURKA and A URKB was studied by confocal microscopy. Results A URKA and A URKB were increasingly expressed from phase G1 and peaked at phase M. After the entrance into mitosis A URKB became the predominant form both in mRNA and protein levels. The proteins of A URKA and A URKB both distributed in the cytoplasm and were associated with nucleus during the first mitosis of mouse zygotes, with some details in different. Conclusion The expression and localization of Aurora kinases A and B was in a cell- cycle regulated manner during the process of the cleavage of mouse zygotes. This discovery will aid in future investigations on their specific roles and molecular mecha- nisms in the regulation of mammalian early embryonic development.

关 键 词：Aurora kinase mouse zygote LOCALIZATION cell cycle regulation 

分 类 号：Q959.837[生物学—动物学] S513[农业科学—作物学]