三七总皂苷和红花总黄酮配伍对急性血瘀大鼠血液流变学的改善作用  

Ameliorative effects of Panax notoginseng saponins combined with Carthamus tinctorius flavonoids on hemorheology in rats with acute blood stasis

作　　者：韩淑燕[1,2] 李海霞[1] 屠鹏飞[1] 

机构地区：[1]北京大学药学院天然药物及仿生药物国家重点实验室,北京100191 [2]北京大学临床肿瘤学院北京肿瘤医院暨北京市肿瘤防治研究所恶性肿瘤发病机制及转化研究教育部重点实验室,北京100142

出　　处：《中国药理学与毒理学杂志》2011年第1期62-67,共6页Chinese Journal of Pharmacology and Toxicology

基　　金：国家杰出青年基金资助课题(30525043)~~

摘　　要：目的观察三七总皂苷(PNS)和红花总黄酮(ECT)配伍对急性血瘀模型大鼠血液流变学指标的影响。方法大鼠按分组分别ig给予PNS 50 mg.kg-1、ECT 200 mg.kg-1、PNS+ECT及阿司匹林100mg.kg-1。每日早晚ig给药各1次,共7次。第5次给药后,sc给予肾上腺素加冰浴造成急性血瘀模型。锥板法测定全血黏度和血浆黏度;微量毛细管法测定血细胞比容(Hct);光电比浊法测定二磷酸腺苷(ADP)诱导的血小板聚集;光电电磁法测定凝血参数。结果与正常对照组相比,模型组大鼠全血黏度和血浆黏度升高,红细胞聚集指数和血小板最大聚集率显著增加,Hct显著升高,纤维蛋白原(Fib)含量显著增加,凝血酶时间(TT)、活化部分凝血活酶时间和凝血酶原时间(PT)均显著缩短(P<0.01)。与模型组相比,单独应用PNS和ECT均能显著降低全血黏度及血浆黏度,明显降低Hct、红细胞聚集指数和血小板最大聚集率,显著延长PT,其中PNS还能显著降低Fib含量,延长TT。与单独应用PNS或ECT相比,PNS+ECT配伍能进一步改善血液流变学指标,在降低Hct上优于单用PNS(P<0.05),对血小板最大聚集率的抑制作用则优于单用PNS或ECT(P<0.05)。与阳性对照阿司匹林相比,单用PNS或ECT抑制血小板聚集的作用不及阿司匹林,但PNS降低Fib的作用较好;PNS+ECT配伍对血液流变学的改善作用与阿司匹林相似,但在抑制红细胞聚集和血小板聚集,降低Fib含量,延长TT和PT等指标上无统计学差异,但有优于阿司匹林的趋势。结论 PNS和ECT单用能显著改善血瘀模型大鼠血液流变学的异常,且二者配伍后能增强对血瘀模型大鼠血液流变学的改善作用。OBJECTIVE To evaluate effects of Panax notoginseng saponins extract（PNS） combined with Carthamus tinctorius extract（ECT） on the hemorheological abnormality in rats with acute blood stasis.METHODS Rats were randomly divided into normal control,model,PNS 50 mg·kg-1,ECT 200 mg·kg-1,PNS 50 mg·kg-1＋ECT 200 mg·kg-1,aspirin 100 mg·kg-1（positive） groups.Rats were ig given corresponding drugs twice a day for consecutive 7 times.After the fifth administration,rats were sc given epinephrine and ice water soaking to set up the acute blood stasis model.Whole blood viscosity and plasma viscosity were evaluated by cone-plate viscometer,hematocrit determined by micro-capillary method,platelet aggregation measured by photoelectric turbidimetry and coagulation parameters evaluated by optical electromagnetic method.RESULTSCompared with normal control group,blood viscosity and plasma viscosity of rats in the blood stasis model group significantly increased;erythrocyte aggregation index（EAI） and the maximum platelet aggregation rate were also remarkably elevated.Meanwhile,the content of hematocrit and fibrinogen（Fib） content was also increased while prothrombin time（PT）,activated partial thrombin time（APTT） and thrombin time（TT） were shortened（P0.01）.Compared with model group,PNS 50 mg·kg-1 or ECT 200 mg·kg-1 alone obviously decreased whole blood viscosity and plasma viscosity,reduced EAI and hematocrit,inhibited the ADP-induced platelet maximum aggregation rate,and prolonged PT（P0.01）.On the other hand,PNS 50 mg·kg-1 significantly decreased Fib content but delayed TT.Compared with each single administration,PNS 50 mg·kg-1＋ECT 100 mg·kg-1 ameliorated abnormal hemorheology in rats with acute blood stasis,as indicated by lower hematocrit（P0.05） and greater platelet maximum aggregation inhibition（compared with PNS group,P0.05）.Compared with aspirin group,PNS and ECT showed less inhibition of platelet aggregation,while PNS had a slightly better effect on Fib content reduction.

关 键 词：三七总皂苷 红花总黄酮 血液流变学 血瘀 血小板聚集 全血凝固时间 纤维蛋白原 

分 类 号：R285.5[医药卫生—中药学]