2000-2009年H1N1甲型流感病毒神经氨酸酶的进化分析和序列解析  

Evolutionary and sequence analysis of neuraminidase of influenza H1N1 in 2000-2009

作　　者：王妮莎[1] 马文丽[1] 危敏[1] 张宝[1] 郑文岭[1] 向星宇[1] 

机构地区：[1]南方医科大学基因工程研究所,广东广州510515

出　　处：《热带医学杂志》2011年第2期155-157,164,共4页Journal of Tropical Medicine

摘　　要：目的研究2000-2009年世界各地不同物种流感病毒神经氨酸酶的进化特征和关键位点的变异情况。结论从NCBI数据库下载所需序列,采用生物信息软件对其氨基酸序列进行种系发生树的构建,以及序列特性的分析。结果新型流感病毒和猪/禽流感病毒NA蛋白在进化关系上非常接近,并且抗原决定簇和糖基化位点的变异也大致相同。结论新型毒株NA蛋白可能由早期的H1N1猪/禽流感病毒进化而来,在进化过程中,一些重要位点的变异导致NA蛋白发生抗原性变异,从而导致流感的大爆发。Objective To study the global evolutionary characteristics and variations of neuraminidase of influenza H1N1 infecting different species during 2000-2009. Method The target sequences were downloaded from NCBI and analyzed using bioinformatic software to construct the phylogenetic tree and analysis the sequences. Result The NA amino acid sequences of novel influenza H1N1 and swine/avian influenza H1N1 are very close in evolutionary relationship,and shared most of the mutated amino acid sites in antigenic sites and glycosylation sites. Conclusion The NA of novel influenza virus might originate from the early swine/avian H1N1 influenza virus, and in the evolutionary process, a number of important sites of NA protein mutated to result in the outbreak of influenza.

关 键 词：H1N1甲型流感病毒 NA 种系发生树 变异位点 

分 类 号：R373.13[医药卫生—病原生物学]