Rice Phospholipase Dαis Involved in Salt Tolerance by the Mediation of H^+-ATPase Activity and Transcription  

作　　者：Peng Shen Rong Wang Wen Jing Wenhua Zhang 

机构地区：[1]College of Life Sciences, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China

出　　处：《Journal of Integrative Plant Biology》2011年第4期289-299,共11页植物学报（英文版）

基　　金：supported by grants from National Natural Science Foundation of China(30625027);from The Ministry of Science and Technology of China (2006CB100100 and 2008AA10Z122);from The Education Department of Jiangsu(200910)

摘　　要：Phospholipase Dα（PLDα） is involved in plant response to salt stress,but the mechanisms remain unclear. We investigated rice PLDα（OsPLDa） localization and its effect on tonoplast（TP） and plasma membrane （PM） H^＋ -ATPase activity and transcription in response to NaCI.When rice suspension-cultured cells were treated with 100 mM NaCI,PLDa activity in cell extracts showed a transient activation with a threefold increase at 1 h.The amount of OsPLDαprotein decreased slightly in the cytosolic fractions,whereas it increased significantly in the TP after NaCI treatment.OsPLDα1 knockdown cells were developed using RNA interference（RNAi） methods.The increase in TP and PM H -ATPase activity induced by NaCI was significantly inhibited in OsPLDα1-RNAi cells.Knockdown of OsPLDα1 prevented the NaCI-induced increase in the transcript level of OsVHA-A（encodes TP H^＋-ATPase） and OSA2（encodes PM H^＋-ATPase）, as well as OsNHX1（encodes TP Na^＋ /H^＋ antiporter）.The cells died more in OsPLDα1-RNAi mutant than in wild type when they were treated with NaCI.These results suggest that OsPLDαis involved in salt tolerance in rice through the mediation of H^＋ -ATPase activity and transcription.Phospholipase Dα（PLDα） is involved in plant response to salt stress,but the mechanisms remain unclear. We investigated rice PLDα（OsPLDa） localization and its effect on tonoplast（TP） and plasma membrane （PM） H^＋ -ATPase activity and transcription in response to NaCI.When rice suspension-cultured cells were treated with 100 mM NaCI,PLDa activity in cell extracts showed a transient activation with a threefold increase at 1 h.The amount of OsPLDαprotein decreased slightly in the cytosolic fractions,whereas it increased significantly in the TP after NaCI treatment.OsPLDα1 knockdown cells were developed using RNA interference（RNAi） methods.The increase in TP and PM H -ATPase activity induced by NaCI was significantly inhibited in OsPLDα1-RNAi cells.Knockdown of OsPLDα1 prevented the NaCI-induced increase in the transcript level of OsVHA-A（encodes TP H^＋-ATPase） and OSA2（encodes PM H^＋-ATPase）, as well as OsNHX1（encodes TP Na^＋ /H^＋ antiporter）.The cells died more in OsPLDα1-RNAi mutant than in wild type when they were treated with NaCI.These results suggest that OsPLDαis involved in salt tolerance in rice through the mediation of H^＋ -ATPase activity and transcription.

分 类 号：S512.1[农业科学—作物学] S188