Peptide actions on oviduct contractions in the large pine weevil, Hylobius abietis  

作　　者：Grzegorz Rosinski Ignacy Korczynski Malgorzata Slocinska Robert Kuzminski 

机构地区：[1]Department of Animal Physiology and Development, Poznen University, Poznan, [2]Department of Forest Entomology, Agricultural University of Poznan, Poznan, Poland

出　　处：《Insect Science》2011年第2期160-165,共6页昆虫科学（英文版）

摘　　要：The peptides proctolin, crustacean cardioactive peptide （CCAP） and FMRFamide, which are known to modulate insect muscle contractions, were assayed for their action on oviduct contractions in Hylobius abietis. A video microscopy technique and computer-based method of data acquisition and analysis were used to investigate the effects of theses peptides on spontaneous contractions of continuously perfused oviducts. All three peptides tested stimulate spontaneous contraction activity of the pine weevil oviduct, increasing the frequency and amplitude of phasic contractions in a dose-dependent manner. Proctolin is more potent as a stimulator than CCAR For proctolin a threshold response of oviduct muscles is at concentration of peptide 10-11-10^-10mol/L and for CCAP at concentration range 10-10-10-9 mol/L. FMRFamide exerts a weak stimulatory effect on the oviduct, and at higher concentrations of the peptide （above 10.8 mol/L）. The peptides exert different responses on oviduct contractions and they may play a role as functional regulators in such processes as egg movement and oviposition.The peptides proctolin, crustacean cardioactive peptide （CCAP） and FMRFamide, which are known to modulate insect muscle contractions, were assayed for their action on oviduct contractions in Hylobius abietis. A video microscopy technique and computer-based method of data acquisition and analysis were used to investigate the effects of theses peptides on spontaneous contractions of continuously perfused oviducts. All three peptides tested stimulate spontaneous contraction activity of the pine weevil oviduct, increasing the frequency and amplitude of phasic contractions in a dose-dependent manner. Proctolin is more potent as a stimulator than CCAR For proctolin a threshold response of oviduct muscles is at concentration of peptide 10-11-10^-10mol/L and for CCAP at concentration range 10-10-10-9 mol/L. FMRFamide exerts a weak stimulatory effect on the oviduct, and at higher concentrations of the peptide （above 10.8 mol/L）. The peptides exert different responses on oviduct contractions and they may play a role as functional regulators in such processes as egg movement and oviposition.

关 键 词：crustacean cardioactive peptide FMRFAMIDE Hylobius abietis oviductcontraction proctolin 

分 类 号：S827[农业科学—畜牧学] Q445[农业科学—畜牧兽医]