依普利酮对大鼠单侧输尿管梗阻模型肾间质纤维化的影响  

Effect of Eplerenone to Fibrosis of Renal Interstitial in Rats Following Unilateral Ureteral Obstruction

作　　者：钟小仕[1] 陈辉[1] 覃丹平[1] 肖笑[1] 刘岩[1] 

机构地区：[1]广东省广州市红十字会医院肾内科(暨南大学医学院第四附属医院),广州510220

出　　处：《中国中西医结合肾病杂志》2011年第2期108-111,190,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：广东省医学科研基金立项项目(No.A2010468);广州市医药卫生科技一般引导项目(No.2009-YB-044)

摘　　要：目的:研究依普利酮对大鼠单侧输尿管梗阻(UUO)模型肾间质纤维化的影响,探讨依普利酮的抗肾间质纤维化作用机制。方法:42只雄性Wistar大鼠随机分成假手术(SO)组、UUO组和UUO+依普利酮治疗组(T-UUO组,依普利酮100mg.kg-1.d-1)。于术后7d、14d分别处死各组7只大鼠。免疫组织化学法测定α-平滑肌肌动蛋白(α-smoothmuscle actin,α-SMA)、单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)、单核细胞/巨噬细胞-1(monocytes/macrophages-1,ED-1),及增殖细胞核抗原(proliferative cell nuclear antigen,PCNA)的表达。结果:UUO组肾间质α-SMA、MCP-1、ED-1、PCNA的表达较SO组增加(P<0.05);在术后各时间点,使用依普利酮治疗的T-UUO组大鼠肾间质α-SMA、MCP-1、ED-1、PCNA的表达较UUO组显著减少(P<0.05),但仍高于SO组(P<0.05)。结论:依普利酮可通过降低α-SMA和MCP-1表达、抑制单核/巨噬细胞浸润和和系膜细胞增生,减轻肾间质纤维化。Objective：To investigate the effect of eplerenone to fibrosis of renal interstitial in rats following unilateral ureteral obstruction,and explore the possible mechanism of anti-fibrosis of renal interstitial.Methods：42 male Wistar rats were distributed casually into sham-operated-groups（SO）and unilateral ureteral obstruction operated groups（UUO）and unilateral ureteral obstruction operation with eplerenone treatment groups（T-UUO,eplerenone 100mg·kg-1·d-1）.Seven rats of each group were killed respectively at 7,14 days after unilateral ureteral obstruction operation.Immunohistochemistry were performed to measure level of expression of a-smooth muscle actin（α-SMA）,monocyte chemoattractant protein-1（MCP-1）,monocytes/macrophages-1（ED-1）,proliferative cell nuclear antigen（PCNA）.Results：The level of expression of interstitial α-SMA、MCP-1、ED-1、PCNA showed a marked increase in UUO when compared to SO（P0.05）.At every time point after operation,in T-UUO were treated by eplerenone,the level of expression of interstitial α-SMA、MCP-1、ED-1、PCNA showed also a marked decrease when compared to UUO（P0.05）.But it still showed increase when compared to SO（P0.05）.Conclusion：Eplerenone inhibit renal interstitial fibrosis by decreasing the level of expression of α-SMA and MCP-1,and suppressess infiltration of monocyte/macrophage and mesangial cell proliferation.

关 键 词：依普利酮 肾间质 纤维化 单侧输尿管梗阻 

分 类 号：R965[医药卫生—药理学]