Characterization of Xanthomonas oryzae- Responsive cis-Acting Element in the Promoter of Rice Race-Specific Susceptibility Gene Xa13  

作　　者：Ting Yuan Xianghua Li Jinghua Xiao Shiping Wang 

机构地区：[1]National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China

出　　处：《Molecular Plant》2011年第2期300-309,共10页分子植物（英文版）

基　　金：This work was supported by grants from the National Natural Science Foundation of China （30930063 and 30921091） and the National Program of Transgenic Variety Development of China （2008ZX08009-004）. No conflict of interest declared.

摘　　要：The rice Xa13 gene, whose promoter harbors a UPT （up-regulated by transcription activator-like [TAL] effector） box, UPTpthXol, plays a pivotal role in the race-specific pathogenicity caused by Xanthomonas oryzae pv. oryzae （Xoo） strain PXO99. PxO99 causes rice disease by inducing Xa13. It is unknown, however, whether the UPTpthXol box is the only PXO99-responsive cis-regulating elements in the activation of Xa13 expression. We analyzed the expression of a series of end- and site-truncated and site-mutated Xa13 promoters in rice and the binding of PXO99 protein to the intact, partial, or site-mutated UPTpthxol boxes. In the Xa13 promoter, UPTpthxol box is the only Xoo-responsive cis-acting element that results in PXO99-induced Xa13 expression. The 5′-terminal second, third, and fourth nucleotides of the box are important for bacterial protein binding and gene activation; mutation of any one of these sites abolished PXO99-induced gene expression. Furthermore, the 3′-half of the UPTpthXol box is also required for protein binding and gene activation. These findings will enhance our understanding of the molecular mechanism of the interaction of rice and Xoo via UPT boxes and TAL effectors.The rice Xa13 gene, whose promoter harbors a UPT （up-regulated by transcription activator-like [TAL] effector） box, UPTpthXol, plays a pivotal role in the race-specific pathogenicity caused by Xanthomonas oryzae pv. oryzae （Xoo） strain PXO99. PxO99 causes rice disease by inducing Xa13. It is unknown, however, whether the UPTpthXol box is the only PXO99-responsive cis-regulating elements in the activation of Xa13 expression. We analyzed the expression of a series of end- and site-truncated and site-mutated Xa13 promoters in rice and the binding of PXO99 protein to the intact, partial, or site-mutated UPTpthxol boxes. In the Xa13 promoter, UPTpthxol box is the only Xoo-responsive cis-acting element that results in PXO99-induced Xa13 expression. The 5′-terminal second, third, and fourth nucleotides of the box are important for bacterial protein binding and gene activation; mutation of any one of these sites abolished PXO99-induced gene expression. Furthermore, the 3′-half of the UPTpthXol box is also required for protein binding and gene activation. These findings will enhance our understanding of the molecular mechanism of the interaction of rice and Xoo via UPT boxes and TAL effectors.

关 键 词：Bacterial blight disease Oryza sativa UPT box Xanthomonas oryzae. 

分 类 号：Q756[生物学—分子生物学] S435.111.4[农业科学—农业昆虫与害虫防治]