毛细管电泳分离和测定吗特灵注射液  

HPCE Determination of Alkaloids of Sophora Flavescens Injection

作　　者：陈亚飞[1] 田颂九[1] 宋景政[1] 孙曾培[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《药物分析杂志》1999年第5期296-299,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的 :建立毛细管电泳法分离和测定吗特灵注射液中槐果碱、苦参碱、氧化槐果碱和氧化苦参碱。方法 :选用缓冲液为 0 2mol·L- 1三羟甲基氨基甲烷 -4 0mmol·L- 1磷酸二氢钠 -2 0 %异丙醇 ,pH 5 5,运行电压 1 2kV ,检测波长 2 0 5nm ,电动进样 5s。含量测定采用双内标 ,内标为重酒石酸间羟胺和西咪替丁。结果 :该法很好地分离了 4种组分 ,线性范围分别为 :槐果碱 0 2～5 7μg·mL- 1,苦参碱 0 3～ 1 6 7μg·mL- 1,氧化槐果碱 1 2～ 59μg·mL- 1,氧化苦参碱 4 8～ 2 4 0μg·mL- 1。结论 :该法分离效率高 ,试剂用量少 ,简便、快速 ,可作为该制剂组分的含量测定方法。Objective:A simple and rapid method was developed for the simultaneous determination of four alkaloid (matrine, oxymatrine, sophocarpine, oxysophocarpine) by capillary zone electrophoresis (CZE) Method:A buffer solution that contained 200 mmol·L -1 Tris salt and 40 mmol·L -1 sodium dihydrogen phosphate and twenty percent isopropanol (pH=5 5) was found to be the most suitable electrolyte for this separation The applied voltage was 12 kV and UV detection wavelength was 205 nm Metaraminol and cimetidine were used as internal standard Results:The linear ranges of the calibration curves were 0 2～240 μg·mL -1 ( r >0 999 1) and detection limits were 0 1～0 3 μg·mL -1 ( S/N =3∶1) Conclusion:The method is highly effective and less solvent consumption and can be applied in rapid quantitative determination of this preparation

关 键 词：高效毛细管电泳 槐果碱 苦参碱 吗特灵 

分 类 号：R284.1[医药卫生—中药学]