甘露糖结合凝集素与人类免疫缺陷病毒感染的关联性研究(英文)  

Association of mannose-binding lectin and HIV-1 infection among the Han Chinese population

作　　者：盛艾娟[1,2] 吴昊[2] 兰洁[3] 路甲鹏[1] 王友信[1] 宋曼殳[1] 武晶晶[1] 刘利新[3] 王嵬[1,3,4] 

机构地区：[1]首都医科大学家庭医学与公共卫生学院,北京100069 [2]首都医科大学附属北京佑安医院 [3]中国科学院研究生院生命科学学院 [4]科廷科技大学健康科学院科廷健康促进研究所

出　　处：《中国病毒病杂志》2011年第2期92-98,共7页Chinese Journal of Viral Diseases

基　　金：国家自然科学基金(30901238);国家"十一五"艾滋病和病毒性肝炎等重大传染病防治科技重大专项项目(2008ZX10001-003,2009ZX10004-903);北京市教育委员会科技发展计划项目(KZ200610025014);北京市属高等学校人才强教深化计划创新团队资助项目(PHR201007112)

摘　　要：目的探讨甘露糖结合凝集素(mannose-binding lectin,MBL)多态性与中国北方汉族人群HIV-1感染的关联。方法采用病例对照研究,以91例HIV感染者(北京佑安医院)和91例健康人(北京同仁医院)作为研究对象,用焦磷酸测序技术(pyrosequencing)检测病例组和对照组MBL ExonⅠ第52、54、57三个密码子的点突变和启动子+4、-221、-550三个位点的多态性,用酶联免疫吸附法(ELISA)检测血浆MBL总浓度及血浆中有活性的MBL浓度,用流式细胞术检测HIV感染者的CD4+T淋巴细胞计数,用bDNA法检测HIV感染者的病毒载量。结果等位基因B在HIV-1感染者中的分布频率显著高于健康对照组(0.18vs0.14)。HIV-1感染者的血浆MBL浓度(0.44)及血浆中活性MBL的浓度(0.61)比健康对照组的浓度要低。携带有A/B、A/C或者B/B基因型的HIV-1感染者与携带有A/A基因型的HIV-1感染者比较,血浆中MBL浓度、血浆中活性MBL浓度以及CD4+T细胞数量均偏低,但病毒载量偏高。结论 MBL与中国汉族人群的HIV-1感染具有关联作用。携带有B等位基因型、低MBL浓度以及低MBL活性浓度的个体,更容易受到HIV-1病毒的感染。Objective Mannose-binding lectin（MBL） is a key molecule of the innate immune system and a component to bind the carbohydrates of a variety of microorganisms,resulting in complement activation and opsonophagocytosis against various pathogens.The study investigated the role of MBL in innate immune responses against HIV-1 infection among the Han Chinese.Methods We randomly selected 91 HIV-1 infected patients from Beijing YouAn Hospital and 91 HIV-1 non-infected healthy individuals from Beijing TongRen Hospital Health Center.Six MBL2 genotypes were analyzed by pyrosequencing for polymorphisms.The method of ELISA was applied for quantitative measurement of serum MBL concentration and MBL complex activity.The CD4＋ T-cell counts were determined by flow cytometry.The plasma viral loads were detected by bDNA method among 91 HIV-1 infected patients.Results The frequency of allele B was higher in HIV-1 infected patients（0.18） than in healthy controls（0.14）.The ratios of median serum MBL concentrations and median MBL complex activities were 0.44∶1 and 0.61∶1 for HIV-1 infected patients to controls,respectively.The HIV-1 infected patients with A/B,A/C or B/B genotypes in exon 1 had higher viral load,lower MBL concentration,lower MBL complex activity and lower CD4＋ T-cell counts than patients with A/A genotype.Conclusions The results suggested the association of MBL with HIV-1 infection among the Han Chinese.The individuals with B variant,low serum MBL concentration and low MBL complex activity might have an increased chance of HIV-1 infection.

关 键 词：人类免疫缺陷病毒(HIV) 甘露糖结合凝集素 SNPS CD4+T细胞计数 病毒载量 

分 类 号：R512.91[医药卫生—内科学] R392.2[医药卫生—临床医学]