大鼠前脊髓综合征的模型制备及评价  

A new model of anterior spinal cord injury syndrome

作　　者：闫金玉[1] 李剑锋[1] 张旭[1] 刘遵南[1] 

机构地区：[1]内蒙古医学院第二附属医院康复医学科,呼和浩特010030

出　　处：《中华物理医学与康复杂志》2011年第4期241-244,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：基金项目：国家自然科学基金资助项目（30860300）

摘　　要：目的 建立大鼠前脊髓综合征模型,并评价其可靠性.方法 选择55只Wistar大鼠,分为假手术组10只和实验组45只;实验组大鼠放置腹侧致伤钩对其腹侧脊髓形成压迫,造成不同程度脊髓损伤,并根据压迫重量和时间分为轻度、中度、重度损伤3个亚组.造模后第1天、第7天、第14天、第4周和第8周采用斜板试验和BBB评分法对大鼠进行行为学评定,并于造模前和造模后第8周检测大鼠体感诱发电位（SEP）和运动诱发电位（MEP）潜伏期.结果 造模后,各实验组斜板试验角度及BBB评分均明显低于假手术组（P＜0.01）,轻、中、重度损伤组之间比较,差异有统计学意义（P＜0.05）;手术第8周,各实验组MEP潜伏期明显高于假手术组（P＜0.05）,各实验组间差异有统计学意义（P＜0.05）;重度损伤组SEP潜伏期明显高于假手术组（P＜0.05）.结论 我们所制备的前脊髓综合征模型出现明显的行为学及神经电生理学改变;根据不同的压迫重量和时间可以制备出不同程度的前脊髓综合征,可作为较为可靠的前脊髓综合征模型.Objective To evaluate a new model of anterior spinal cord injury （SCI） syndrome and to explore the pathophysiology of SCI. Methods Fifty-five Wistar rats were randomly divided into a sham-operated group （ 10 rats） and an experimental group （45 rats）. A metal hook was fixed in front of the rats＇abdomens to compress the ventral part of the spinal cord in the experimental group. According to the degree and time of compression,the rats in the experimental group were divided into light, moderate, heavy injury subgroups. The tilt board test and the Bosso-Beattie- Bresnahan （BBB） locomotor rating scale were used to assess the rats＇ behavior at the 1st , 7th andl4th days and after 4 and 8 weeks. The latency of somatosensory evoked potential （SEP） and motor evoked potential （MEP） were measured before and 8 weeks after the operation. Results After the operation the gradients in the tilt board test and BBB scores in the experimental subgroups were all significantly lower than in the sham-operated group. There were also significant differences among the 3 severity subgroups. Eight weeks after the operation the average MEP latencies in the experimental subgroups were significantly longer than in the sham-operated group, and there were also significant differences among the 3 severity sub-groups. MEP in the heavy injury subgroup was significantly longer compared with the sham-operated group. Conclusions Obvious behavioral and neuroelectrophysiological changes were observed in the injured rats. Models of different severity could be prepared and reproduced to simulate clinical SCI.

关 键 词：前脊髓综合征 模型 体感诱发电位 运动诱发电位 WISTAR大鼠 

分 类 号：R687.3[医药卫生—骨科学]