低强度电磁场促进鼠表皮干细胞移植修复皮肤缺损的实验研究  

The effects of low intensity electromagnetic fields on the reconstruction of full skin loss wounds grafted with human epidermal stem cells

作　　者：张鸣生[1] 吴博[1] 白文芳[1] 庞文君[1] 朱洪翔[1] 许伟成[1] 

机构地区：[1]广东省人民医院(广东省医学科学院)老年医学研究所,广州510080

出　　处：《中华物理医学与康复杂志》2011年第4期250-253,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：基金项目：广东省自然科学基金（9151008901000199）

摘　　要：目的 观察低强度电磁场（LIEMF）在促进表皮干细胞（ESCs）移植修复皮肤缺损中的作用.方法 取7～8周龄的裸鼠50只,制作全层皮肤缺损后根据随机数字表随机分为3个实验组（1 Hz组、10 Hz组和50 Hz组）和2个对照组（细胞悬液对照组和空白对照组）,每组10只.3个实验组和细胞悬液对照组利用胶原海绵将体外分离培养的人包皮来源的ESCs植入裸鼠创面,实验组外加LIEMF（磁感应强度5 mT,频率分别为1 Hz、10 Hz和50 Hz）刺激15 d（每日1次,每次30 min）;空白对照组置于同等条件下不加细胞悬液和电磁场.通过计算创面收缩率,光镜下观察组织切片HE染色和免疫组织化学染色结果,以及透射电镜下观察再生皮肤组织内部结构,评定皮肤缺损修复情况.结果 ESCs可以在裸鼠皮肤缺损处成功生长,再生表皮层中有人β1整合素强阳性表达,并且实验组创面收缩率均高于对照组（P＜0.05）.光镜和电镜下观察到,LIEMF干预后,各实验组再生皮肤具备完整的表皮层和真皮层,细胞层次增多,表皮层明显增厚,附件丰富,表皮细胞间有桥粒、半桥粒、粗张力微丝.结论 LIEMF可以促进移植了人表皮干细胞的裸鼠全层皮肤缺损创面愈合.Objective To observe the effects of low intensity electromagnetic fields （LIEMFs） in promoting the reconstruction of full skin loss wounds grafted with human epidermal stem cells （ESCs）. Methods Fifty nude mice aged 7 to 8 weeks with full skin loss wounds were equally divided into 3 experimental subgroups （ 1 Hz, 10Hz and 50Hz） and two control groups （a cell suspension control group and a blank control group） , with ten mice each. In the 3 experimental subgroups and the cell suspension control group, ESCs separated from human foreskin and cultured in vitro were grafted to the wounds using collagen sponge scaffolds. The experimental subgroups were then stimulated with an LIEMF （magnetic field intensity 5mT） at the appropriate frequency for 30min/day for 15 days. The blank control group was put under the same conditions without the cell suspension and LIEMF. The healing rates of the wounds were observed, and tissue slices were stained and observed under a light microscope. The inner structure of the regenerating skin was observed using transmission electron microscopy. Results The ESCs were successfully grafted. A few human integrin β1 positive stained cells appeared in the regenerating skin. The average healing rates in the experimental subgroups were significantly superior to those of the control groups. Well differentiated epidermis and dermis could be seen in the regenerating skin in all of the experimental groups. The epidermis had more cell layers and was thicker than in the control groups. More desmosome, hemidesmosome and keratin filaments were seen among the epidemic cells of the experimental groups. Conclusions LIEMF promotes the healing of full skin loss wounds grafted with ESCs in nude mice, and can promote complete repair of skin defects and the regeneration of skin function.

关 键 词：低强度电磁场 表皮干细胞 移植 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]