微流控芯片精子分选法对精子常规参数及DNA完整性的影响  

Effects of a microfluidic sperm sorter on sperm routine parameters and DNA integrity

作　　者：王维[1] 梁广铁[1] 彭娅娅[1] 刘大渔[1] 周小棉[1] 

机构地区：[1]广州医学院附属广州市第一人民医院检验科,广东广州510180

出　　处：《中华男科学杂志》2011年第4期301-304,共4页National Journal of Andrology

基　　金：国家自然科学基金(30870753);广东省自然基金(9151008901000190);广州市医药卫生科技重点项目(2009-ZDi-10;2009-ZDi-21);广州市医药卫生科技项目(2009-YB-033);广东省医学科研基金(A2010458);广东省人口和计划生育委员会重点课题(2009104)~~

摘　　要：目的:研究微流控芯片精子优选技术对精子常规参数及DNA完整性的影响。方法:自行设计制作微流控芯片,利用芯片处理技术和上游法对40例精液标本进行精子优选,通过计算机辅助精液分析系统及染色质扩散试验从精子常规参数及DNA完整性两个方面评价精液体外处理对精子的影响。结果:精液经微流控芯片法和上游法处理后,精子活力、精子正常形态率以及精子尾部肿胀率均有显著提高(P<0.01),精子的DNA损伤率明显降低(P<0.01)。微流控芯片法与上游法相比,优选后前者精子DNA损伤率明显低于后者[(8.4±5.8)%vs(16.4±9.2)%,P<0.01],而其他参数差异无显著性。结论:微流控芯片技术在精子优选中能获得精子DNA损伤程度小的高质量精子。Objective： To investigate the effects of a microfluidic sperm sorter on the routine parameters and DNA integrity of human sperm. Methods： We divided 40 semen samples into two aliquots and performed sperm sorting using a self-made polydimethylsiloxane microfluidic sperm sorter and the swim-up method, respectively. Then we evaluated and compared the effects of these two methods on the sperm routine parameters and DNA integrity by computer-assisted sperm analysis and sperm chromatin disper- sion test. Results ： After processing, sperm motility, normal morphology and tail hypoosmotic swelling rate were significantly improved, while sperm DNA damage remarkably decreased （ P 〈 0.01 ）. The mierofluidic sperm sorter achieved a significantly lower rate of sperm DNA damage than the swim-up method （ [ 8.4 ± 5.8 ] % vs [ 16.4 ±9.2 ] %, P 〈 0.01 ）, but no statistically significant differences were found in all other parameters between the two methods. Conclusion： High-quality sperm with less DNA integrity damage could be obtained in sperm sorting with the microfluidic sperm sorter.

关 键 词：微流控芯片 上游法 精子优选 染色体扩散试验 DNA完整性 

分 类 号：R714.8[医药卫生—妇产科学]