胁迫诱导谷胱甘肽还原酶活性和同工酶谱的变化与H_2O_2的关系(英文)  

Relationship between H_2O_2 and Changes of Glutathione Reductase Activity and Isoenzyme Pattern Induced by Stress

作　　者：赵凤云[1] 胡凡[1] 韩明明[1] 刘文[1] 刘涛[1] 

机构地区：[1]山东理工大学生命科学学院,山东淄博255049

出　　处：《西北植物学报》2011年第3期543-551,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金资助项目(30671126)

摘　　要：以多种胁迫处理的野生型水稻中花11及其携带谷胱甘肽转移酶(GST)和过氧化氢酶(CAT)的功能获得突变体为材料,分析地上部H2O2含量、谷胱甘肽还原酶(GR)活性和同工酶谱的变化.结果显示,胁迫条件下野生型水稻(W)中H2O2的含量明显高于其突变体(M).非胁迫条件下W和M的GR同工酶都有3条带,且W和M之间无显著差异.单一镉、PEG-6000和外源H2O2处理诱导W均产生1条额外谱带,用H2O2和抗坏血酸(AsA)同时处理时,W中则未见该条带.当用镉和CAT抑制剂复合处理时,W和M的所有同工酶活性都降低;而同时用镉和H2O2清除剂处理时,W中则无GR同工酶条带.在其他处理(盐、高温、Zn、盐+高温、镉+高温、PEG+高温和Cd+Zn)条件下,W和M的GR活性和/或同工酶谱的变化也有明显差别.在所有处理中,W和M的GR总酶活性的变化与其同工酶的活性变化基本一致.在胁迫条件下W和M积累H2O2的水平不同,其GR活性和/或同工酶谱的变化也存在明显差异.研究表明,不同环境胁迫诱导水稻GR活性和同工酶谱的变化可能与H2O2水平有关.The changes of H2O2 content,GR activity and isoenzyme pattern were studied by using wild type（Oryza sativa L.cv ‘Zhonghua 11＇,W） and the gain-of-function mutant（M） of rice（co-expressing glutathione-S-transferase（GST,EC.2.5.1.18） and catalase1（CAT1,EC.1.11.1.6）） treated with multiple abiotic stress.The results showed that the content of H2O2 in W is significantly higher than that in M under all stresses.Three isoenzymes of GR were observed both in W and M under non-stressed conditions,and there was no significant difference between W and M.An extra isoenzyme was induced by cadmium（Cd）,PEG-6000 and exogenous H2O2 in W,respectively.However,this band canot be induced in W by combined H2O2 and ascorbate.When subjected to the combined Cd and 3-amino-1,2,4-triazole（a CAT inhibitor）,the activities of GR isoenzymes decreased in both W and M,but no GR isoforms were observed in W following Cd together with N,N′-dimethylthiourea（a scavenger of H2O2） application.Moreover,the GR isoenzyme patterns and/or activities with other applied stresses were also significantly different（salt,zinc,heat,heat＋salt or Cd or PEG,zinc＋Cd） in W and M.The total GR activity consisted with the increased/decreased expression of GR isoenzymes in W and M under all treatments.These results indicated that the variations of GR activity and isoenzymes under single and combined abiotic stresses in rice may be related to the level of H2O2.

关 键 词：环境胁迫 过氧化氢酶 功能获得突变体 谷胱甘肽转移酶 谷胱甘肽还原酶 

分 类 号：Q945.78[生物学—植物学]