漆树8个品种和5个野生居群HPLC指纹图谱分析  

HPLC fingerprint analysis of Eight Varieties and Five Wild Populations of Toxicodendron vernicifluum

作　　者：魏朔南[1] 李林[1] 赵喜萍[1] 赵猛[1] 胡正海[1] 

机构地区：[1]西北大学生命科学学院,西安710069

出　　处：《西北植物学报》2011年第3期620-627,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30870136);陕西省自然科学基金(ST08-ZT01)

摘　　要：采用HPLC指纹图谱技术分析了陕西漆树8个品种和5个野生居群的树皮提取物化学成分,并以提取物中6个化学成分含量为变量进行聚类分析.结果表明:(1)通过对2、3、4和5号主要特征峰分析,漆树8个品种和5个野生居群的13个样品平均相似度为0.974;方差和变异系数分析显示,各主要特征峰含量、漆酚总量之间存在差异,且变异系数最大达61.49%.(2)聚类分析结果显示,依据漆酚总量等6个变量完全可将漆树8个品种的8个样品分开,表明单烯漆酚为区分8个样品的第一关键因子,未知化合物和二烯漆酚为第二、第三关键因子.同时,采自商南的2个漆树品种样品和采自平利的6个品种样品分别聚为两类,表明其聚类结果与漆树地理分布存在密切关系.The HPLC fingerprinting method was used to analyze the chemical components which were extracted from 8 varieties and 5 wild population lacquer tree barks.At the same time,according to the studying of 6 components extracted from those samples,the clustering analysis method was used to treat the data.The results showed that average similarity of 13 samples were over 0.974 based on the analysis of No.2,3,4 and 5 major characteristic peaks.From the analysis of variance and coefficient of variance（CV）,the various major characteristics peaks and the total amount of urushiol were distinct and the maximum of CV was 61.49%.The 8 samples from 8 varieties of the lacquer tree could be distinguished completely by the clustering analysis.The first key factor was the monoene urushiol;the unknown compound and the diene urushiol were the second and the thired key factors.The clustering results have a close relationship with the geographic distribution of the lacquer tree.Two varieties of the lacquer tree from Shangnan and 6 varieties from Pingli were clustered to 2 categories.

关 键 词：漆树 HPLC 指纹图谱 漆酚 品种 

分 类 号：Q503[生物学—生物化学]