白藜芦醇体外抗肠道病毒71型的作用  

Inhibitory effect of resveratrol against enterovirus type 71 in vitro

作　　者：高璐璐[1,2] 陈清[1] 李建栋[1] 朱文昌[1] 郭江[1] 俞守义[1] 

机构地区：[1]南方医科大学热带医学与公共卫生学院流行病学系,广东广州510515 [2]广州军区空军后勤部卫生防疫队,广东广州510620

出　　处：《南方医科大学学报》2011年第4期665-668,共4页Journal of Southern Medical University

基　　金：卫生部"十一五"科技重大专项(20092X10001-018)

摘　　要：目的在体外观察白藜芦醇抗肠道病毒71的作用效果。方法将白藜芦醇作用于Vero细胞,CCK-8法检测其细胞毒性。以利巴韦林作为对照药物,分别在病毒感染细胞的不同阶段加入白藜芦醇,观察细胞病变(CPE),CCK-8法检测并计算病毒抑制率、半数有效浓度(IC50)及选择指数(SI),评价白藜芦醇的抗病毒效果。结果低浓度的白藜芦醇对细胞是无毒性的,细胞半数毒性浓度(TC50)为307.6 mmol/L。白藜芦醇只在预处理细胞后具备抗病毒效果,药物半数有效浓度(IC50)为20.2 mmol/L,白藜芦醇抗EV71的选择指数(SI)为15.2。而在病毒吸附细胞后,以及白藜芦醇直接与病毒液混合,均无抗病毒作用。结论白藜芦醇具有一定的抗肠道病毒71的作用。Objective To observe the inhibitory effect of resveratrol against the cytopathogenicity of enterovirus type 71.Methods The cytotoxicity of resveratrol on Vero cells was detected using cell counting kit-8（CCK-8）.The antiviral activity of resveratrol in different stages of infection,with ribavirin as the control,was evaluated by determining the virus inhibition rate,medium effective concentration（IC50）,and selection index（SI）.Results Resveratrol was nonpoisonous to Vero cells with an median toxic concentration（TC50） of 307.6 mmol/L.Resveratrol produced an obvious inhibitory effect against enterovirus type 71 only before the cell infection by the virus（IC50=20.2 mmol/L,SI=15.2）,and once the cells were infected,resveratrol no longer had such antiviral effect.Conclusion Resveratrol may offer some protection against enterovirus type 71 in vitro.

关 键 词：白藜芦醇 肠道病毒71型 体外 

分 类 号：R285[医药卫生—中药学]