乙型肝炎病毒聚合酶与UXT蛋白的潜在相互作用鉴定  

Identification of ubiquitously expressed transcript as the potential interactor of hepatitis B virus polymerase

作　　者：陈孟璋[1] 邹奕[1] 李君武[1] 李月琴[1] 李弘剑[1] 周天鸿[1] 

机构地区：[1]暨南大学生命科学技术学院生物工程学系,广东广州510632

出　　处：《南方医科大学学报》2011年第4期686-689,共4页Journal of Southern Medical University

基　　金：国家科技重大专项课题(2008ZX10002-009)~~

摘　　要：目的研究乙肝病毒聚合酶(HBV Pol)在肝细胞内潜在的相互作用的蛋白质因子,为揭示HBV Pol发挥功能的分子机制,进一步了解HBV在细胞内复制的机制提供依据。方法构建重组诱饵质粒pGBKT7-Pol,利用GAL4酵母双杂交系统筛选人肝cDNA文库得到与HBV Pol相互作用的蛋白质因子,再通过GST-Pull down验证蛋白间的相互作用。结果酵母双杂交筛选得到宿主蛋白Ubiquitously Expressed Transcrip(tUXT),GST-pull down确证蛋白间的相互作用。结论 UXT为一潜在的与HBV Pol相互作用的肝细胞蛋白质因子,这可能为研究HBV Pol在病毒生活周期中的功能提供线索。Objective To investigate the function of hepatitis B virus polymerase（HBV Pol） in the viral life cycle by screening the proteins interacting with HBV polymerase.Methods The HBV Pol gene was constructed into the pGBKT7 vector.GAL4 yeast two-hybrid system was used to screen the human liver cDNA library to obtain proteins which interacted with HBV Pol.GST-pull down assay was applied to confirm the protein interactions.Results Ubiquitously expressed transcript（UXT） was selected by the yeast two-hybrid system.GST-pull down assay confirmed the in vitro interaction between HBV Pol and UXT.Conclusion UXT is a potential interactor of HBV Pol,and this protein interaction may provide clues of the function of HBV Pol in HBV life cycle.

关 键 词：酵母双杂交 乙肝病毒聚合酶 UXT 蛋白质相互作用 

分 类 号：Q939.4[生物学—微生物学] Q78