反相高效液相色谱法测定根皮苷原料药的含量  

Determination of the content of phloridzin using reversed-phase high-performance liquid chromatography

作　　者：贺建荣[1] 刘雪英[2] 王庆伟[1] 叶子晨[3] 畅敏[1] 

机构地区：[1]第四军医大学唐都医院药剂科,陕西西安710038 [2]第四军医大学药学系药物化学教研室,陕西西安710033 [3]第四军医大学药学系学员队,陕西西安710033

出　　处：《南方医科大学学报》2011年第4期711-713,共3页Journal of Southern Medical University

基　　金："重大新药创制"科技重大专项项目(2011ZX09401-308-19)

摘　　要：目的采用反相高效液相色谱法测定根皮苷原料药含量,以满足质量控制的要求。方法采用RP-HPLC法,色谱柱为Inertsil ODS-3柱(4.6×150 mm,5μm)。流动相为乙腈-0.15%磷酸(25∶75),柱温30℃,流速1.0 ml/min,检测波长285nm。结果此条件下根皮苷分离良好,在0.4950～49.50μg·mL-1浓度范围内线性关系良好,回归方程为:Y=46.370X-0.673(r=0.9999,n=3),平均回收率为99.40%,相对标准偏差为0.67%。结论建立根皮素原料药含量测定的HPLC方法专属性强、灵敏度高,为根皮素质量控制提供参考依据。Objective To establish a reversed-phase high-performance liquid chromatographic（RP-HPLC） method for determination of phloridzin content.Methods A RP-HPLC method was established for determination of phloridzin using an Inertsil ODS-3（4.6×150 mm,5 μm） column with the detection wavelength of 288 nm,flow rate of 1.0 ml/min,and column temperature of 25 ℃.Results The result showed that the phloridzin had a good linear relationship when its concentration ranged between 0.5988 and 89.72 μg/ml.The regression equation was Y=46.370 X-0.6728（r=0.9999,n=3）.The average recovery of phloridzin was 99.40% with the relative standard deviations（RSD） of 0.67%.Conclusion This method is simple,quick and accurate for determination of phloridzin content.

关 键 词：反相高效液相色谱法 根皮苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]