Analysis of the urinary peptidome associated with Helicobacter pylori infection  

作　　者：Di Xiao Fan-Liang Meng Li-Hua He Yi-Xin Gu Jian-Zhong Zhang 

机构地区：[1]National Institute for Communicable Disease Controland Prevention, Chinese Center for Disease Control and Pre-vention, Beijing 102206, China

出　　处：《World Journal of Gastroenterology》2011年第5期618-624,共7页世界胃肠病学杂志（英文版）

基　　金：Supported by The National Science and Technology Pillar Program of the Ministry of Science and Technology of the People’s Republic of China during the Eleventh Five-Year plan period,No. 2007BAID4B02

摘　　要：AIM： To investigate the relationship between urinary peptide changes and Helicobacter pylori （H. pylorl） infection using urinary peptidome profiling. METHODS： The study was performed in volunteers （n = 137） who gave informed consent. Urinary peptides were enriched by magnetic beads based weak cation exchange chromatography＇and spectrums acquired by matrix-assisted laser desorption/ionization time-of-flight （MALDI-TOF） mass spectrometry （MS）. ClinProTools bioinformatics software was used for statistical analysis and the recognition of peptide patterns. The marker peptides were identified by LTQ Obitrap XL tandem MS. RESULTS： Approximately 50 proteins or peptides which loaded onto the magnetic beads were detected by MAL-DI-TOF MS. By optimizing the parameters of the model, the Genetic Algorithm model had good recognition capability （97%） and positive predictive value （94%）. Based on the model, 2 markers with molecular masses of 6788 and 1912 Da were found that differentiated between H. pylori positive and negative volunteers. The m/z 1912 sequence was parsed as SKQFTSSTSYN- RGDSTF. The peptide was identified as isoform 1 of the fibrinogen a chain precursor, whose concentration in urine was markedly higher in H. pylori infected volunteers than in H. pylori non-infected ones. CONCLUSION： The appearance of urinary fibrinogen degradation products is caused by an active H. pyloriinduced process.AIM:To investigate the relationship between urinary peptide changes and Helicobacter pylori(H.pylori) infection using urinary peptidome profiling.METHODS:The study was performed in volunteers(n = 137) who gave informed consent.Urinary peptides were enriched by magnetic beads based weak cation exchange chromatography and spectrums acquired by matrix-assisted laser desorption/ionization time-of-flight(MALDI-TOF) mass spectrometry(MS).ClinProTools bioinformatics software was used for statistical analysis and the recognition of peptide patterns.The marker peptides were identified by LTQ Obitrap XL tandem MS.RESULTS:Approximately 50 proteins or peptides which loaded onto the magnetic beads were detected by MALDI-TOF MS.By optimizing the parameters of the model,the Genetic Algorithm model had good recognition capability(97%) and positive predictive value(94%).Based on the model,2 markers with molecular masses of 6788 and 1912 Da were found that differentiated between H.pylori positive and negative volunteers.The m/z 1912 sequence was parsed as SKQFTSSTSYNRGDSTF.The peptide was identified as isoform 1 of the fibrinogen α chain precursor,whose concentration in urine was markedly higher in H.pylori infected volunteers than in H.pylori non-infected ones.CONCLUSION:The appearance of urinary fibrinogen degradation products is caused by an active H.pyloriinduced process.

关 键 词：Urinary peptidome profiling MB-matrix-assisted laser desorption/ionization time-of-flight massspectrometry Helicobacter pylori Fibrinogen degrada-tion products 

分 类 号：O633.14[理学—高分子化学] Q785[理学—化学]