Influence of CXCR4/SDF-1 axis on E-cadherin/β-catenin complex expression in HT29 colon cancer cells  

作　　者：Lin Wang Cui-Ling Li Lei Wang Wen-Bin Yu Hai-Peng Yin Guang-Yong Zhang Li-Feng Zhang Sheng Li San-Yuan Hu 

机构地区：[1]Department of General Surgery,Qilu Hospital, Shandong University, Jinan 250012, ShandongProvince, China [2]Key Laboratory for Modem Medi-cine and Technology of Shandong Province, Institute of BasicMedicines, Shandong Academy of Medical Sciences, Jinan250062, Shandong Province, China [3]Depatment of Hepatobiliary Surgery, Shandong Tu-mor Hospital, Jinan 250117, Shandong Province, China

出　　处：《World Journal of Gastroenterology》2011年第5期625-632,共8页世界胃肠病学杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China,No. 30571712 and 30810403081

摘　　要：AIM： To study the influence of CXCR4/stromal cell- derived factor-1 （SDF-1） axis on E-cadherin/β-catenin complex expression in HT29 colon cancer ceils and its underlying mechanisms. METHODS： Effect of SDF-1 on E-cadherin/β-catenin expression was detected by immunocytochemistry. E-cadherin and/3-catenin mRNA expression levels were measured by reverse transcriptase-polymerase chain reaction. SDF-l-induced phosphorylation of phosphati- dylinositol 3-kinase （PI3K）/AKT and β-catenin was detected by Western blotting. RESULTS： The E-cadherin and β-catenin mRNA ex-pression levels in HT29 cells were lower 48 h after incubated with SDF-1 at the concentrations of 20 and 40 ng/mL （P 〈 0.05）. SDF-l-induced significant phosphorylation of PI3K/AKT and β-catenin. AMD3100 and LY294002 inhibited the phosphorylation of PI3K/AKT and β-catenin. CONCLUSION： SDF-1 down-regulates the E-cadherin/ β-catenin complex expression in HT29 cells by decreasing mRNA synthesis and increasing β-catenin phosphorylation.AIM:To study the influence of CXCR4/stromal cellderived factor-1(SDF-1) axis on E-cadherin/β-catenin complex expression in HT29 colon cancer cells and its underlying mechanisms.METHODS:Effect of SDF-1 on E-cadherin/β-catenin expression was detected by immunocytochemistry.E-cadherin and β-catenin mRNA expression levels were measured by reverse transcriptase-polymerase chain reaction.SDF-1-induced phosphorylation of phosphatidylinositol 3-kinase(PI3K)/AKT and β-catenin was detected by Western blotting.RESULTS:The E-cadherin and β-catenin mRNA expression levels in HT29 cells were lower 48 h after incubated with SDF-1 at the concentrations of 20 and 40 ng/mL(P < 0.05).SDF-1-induced significant phosphorylation of PI3K/AKT and β-catenin.AMD3100 and LY294002 inhibited the phosphorylation of PI3K/AKT and β-catenin.CONCLUSION:SDF-1 down-regulates the E-cadherin/β-catenin complex expression in HT29 cells by decreasing mRNA synthesis and increasing β-catenin phosphorylation.

关 键 词：CXCR4 Stromal cell-derived factor-i E-cad-herin Β-CATENIN Phosphatidylinositol 3-kinase/AKT Co-lon cancer 

分 类 号：Q617[生物学—生物物理学] Q279