Probiotic Lactobacillus rhamnosus downregulates FCER1 and HRH4 expression in human mast cells  

作　　者：Anna Oksaharju Matti Kankainen Riina A Kekkonen Ken A Lindstedtt Petri T Kovanen Riitta Korpela Minja Miettinen 

机构地区：[1]Wihuri Research Institute [2]VTT Technical Research Centre of Finland,Biosystems Modelling [3]Valio Ltd,Research Centre [4]University of Helsinki,Institute of Biomedicine,Medical Nutrition Physiology [5]National Institute for Health and Welfare,Department of Vaccines and Immune Protection

出　　处：《World Journal of Gastroenterology》2011年第6期750-759,共10页世界胃肠病学杂志（英文版）

基　　金：Supported by Valio Research Centre,the Foundation for Nutrition Research,Academy of Finland Research Council for Biosciences and Environment,Grant No.129954;Finnish Funding Agency for Technology and Innovation (TEKES) grant No.2243/31/05

摘　　要：AIM:To investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis.METHODS:Human peripheral-blood-derived mast cells were stimulated with Lactobacillus rhamnosus (L.rhamnosus) GG (LGG),L.rhamnosus Lc705 (Lc705),Propionibacterium freudenreichii ssp.shermanii JS (PJS) and Bifidobacterium animalis ssp.lactis Bb12 (Bb12) and their combination for 3 or 24 h,and were subjected to global microarray analysis using an Affymetrix GeneChip Human Genome U133 Plus 2.0 Array.The gene expression differences between unstimulated and bacteria-stimulated samples were further analyzed with GOrilla Gene Enrichment Analysis and Visualization Tool and MeV Multiexperiment Viewer-tool.RESULTS:LGG and Lc705 were observed to suppress genes that encoded allergy-related high-affinity IgE receptor subunits α and γ (FCER1A and FCER1G,respectively) and histamine H4 receptor.LGG,Lc705 and the combination of four probiotics had the strongest effect on the expression of genes involved in mast cell immune system regulation,and on several genes that encoded proteins with a pro-inflammatory impact,such as interleukin (IL)-8 and tumour necrosis factor alpha.Also genes that encoded proteins with anti-inflammatory functions,such as IL-10,were upregulated.CONCLUSION:Certain probiotic bacteria might diminish mast cell allergy-related activation by downregulation of the expression of high-affinity IgE and histamine receptor genes,and by inducing a pro-inflammatory response.AIM：To investigate the effects of four probiotic bacteria and their combination on human mast cell gene expression using microarray analysis.METHODS：Human peripheral-blood-derived mast cells were stimulated with Lactobacillus rhamnosus （L.rhamnosus） GG （LGG）,L.rhamnosus Lc705 （Lc705）,Propionibacterium freudenreichii ssp.shermanii JS （PJS） and Bifidobacterium animalis ssp.lactis Bb12 （Bb12） and their combination for 3 or 24 h,and were subjected to global microarray analysis using an Affymetrix GeneChip Human Genome U133 Plus 2.0 Array.The gene expression differences between unstimulated and bacteria-stimulated samples were further analyzed with GOrilla Gene Enrichment Analysis and Visualization Tool and MeV Multiexperiment Viewer-tool.RESULTS：LGG and Lc705 were observed to suppress genes that encoded allergy-related high-affinity IgE receptor subunits α and γ （FCER1A and FCER1G,respectively） and histamine H4 receptor.LGG,Lc705 and the combination of four probiotics had the strongest effect on the expression of genes involved in mast cell immune system regulation,and on several genes that encoded proteins with a pro-inflammatory impact,such as interleukin （IL）-8 and tumour necrosis factor alpha.Also genes that encoded proteins with anti-inflammatory functions,such as IL-10,were upregulated.CONCLUSION：Certain probiotic bacteria might diminish mast cell allergy-related activation by downregulation of the expression of high-affinity IgE and histamine receptor genes,and by inducing a pro-inflammatory response.

关 键 词：Probiotic bacteria Mast cells MICROARRAY ALLERGY IgE receptor 

分 类 号：R371[医药卫生—病原生物学]