榄香烯复合瘤苗主动免疫抗瘤效应增强途径的比较研究  

Comparative Study on the Active Immunization Antitumor Effects Strengthen Way of Elemene-Combo Tumor Cell Vaccine

作　　者：金梅[1] 施广霞[2] 朴花[2] 薛冰[2] 钱振超[2] 

机构地区：[1]辽宁师范大学生命科学院,116029 [2]大连医科大学病理生理学教研室,116027

出　　处：《大连医科大学学报》1999年第3期166-171,共6页Journal of Dalian Medical University

基　　金：国家自然科学基金

摘　　要：将ＢＡＬＢ／ｃ 小鼠的Ｈ２２ 腹水型肝癌和６１５ 系小鼠的Ｌ６１５ 白血病细胞经榄香烯或／和热休克等不同处理制成瘤苗， 进行体内主动免疫实验和致敏脾细胞的体外细胞毒活性实验。结果表明： 热休克和榄香烯复合瘤苗比单一因素处理的瘤苗免疫效果好， 经Ｈ２２和Ｌ６１５ 复合瘤苗免疫、攻击后两个月动物存活率分别达６０－０％ 、８５－０％ （ Ｐ＜０－０１）， 并且能够明显提高死亡小鼠的平均存活天数。瘤苗免疫动物脾细胞的细胞毒活性分别达５８－８％ 、６９－７％ （ Ｐ ＜ ０－０１） 。在制备复合瘤苗时先加入榄香烯后再热休克的免疫效果较好， 这在Ｌ６１５ 瘤苗更为明显， 从而为今后瘤苗免疫在临床上的应用提供了进一步的实验依据。Tumor cell vaccine(TCV)of H 22 ascitic hepatoma and L615 leukemia that were treated with ele mene or/and heat shock was studied both in vivo active immunoprophylaxis experiment and in vitro cytotoxic activity test of immune spleen cells.The results showed that heat shock and elemene-combo tumor cell vaccine had better immunoprotective effect than that of TCVs treated with heat shock or elemene alone.Two months after challenged,the survival rate of H 22 -combo TCV and L615-combo TCV immunized mice reached 60.0% and 85.0% respectively(P<0.01),and the cytotoxic activity of the TCV immunized spleen cells reached 58.8%and 69.7% respectively(P<0.01).The mean survival time(MST)of TCV immunized mice was also significantly prolonged.It seemed that the immunoprotective effect of combo-TCV was better in case of first adding elemene than that of first heat shock which was obvious in L615 tumor cell vaccine experiment.This study might further provide some experimental basis for the clinical application of tumor cell vaccine active immunization.

关 键 词：热休克 榄香烯 瘤苗 H22肝癌 L615白血病 

分 类 号：R392－33[医药卫生—免疫学] R979.19[医药卫生—基础医学]