春石斛兰愈伤组织的诱导及分化培养基的筛选  

Selection of Medium of Induction and Differentiation of Callus of Dendrobium nobile lindl

作　　者：张爱香[1] 左利兵 刘会清[1] 常美花[1] 

机构地区：[1]河北北方学院农林科技学院,河北张家口075131 [2]张家口市蔬菜技术推广站,河北张家口075000

出　　处：《河北北方学院学报（自然科学版）》2011年第1期36-37,共2页Journal of Hebei North University:Natural Science Edition

基　　金：河北省科技厅资助项目(052201122)

摘　　要：以春石斛兰无菌幼苗茎段为外植体,分别研究了愈伤组织的诱导、分化、生根培养基配方.结果表明,愈伤诱导培养基为MS+6-BA 0.5 mg/L+2.4-D 0.8 mg/L;愈伤分化率最高的培养基为MS+NAA1.2mg/L+KT1.5 mg/L,不定芽生根培养基为1/2MS或者MS+NAA0.5 mg/L.Taking stem segments from seeding of Dendrobium nobile lindl eucalypts as explants,study on culture medium of callus induction,differentiation and rooting was done in our experiment.The result showed that the culture medium of callus induction was MS culture medium containing 0.5 mg/L 6-BA and 0.8 mg/L 2.4-D.Using MS culture medium containing 1.2 mg/L NAA and KT 1.5 mg/L,differentiation rate of callus was the maximum.Culture medium for rooting of adventitious bud was 1/2 MS culture medium or MS culture medium containing 0.5 mg/L NAA.

关 键 词：春石斛兰 组织培养 愈伤 分化 生根 

分 类 号：S682.310.35[农业科学—观赏园艺]