外源性Apelin-13对心力衰竭大鼠血浆血管紧张素Ⅱ和肾上腺髓质素的影响  

Effect of Exogenous Apelin-13 on Expression of Plasma Angiotensin-Ⅱ and Adrenomedulin in Heart Failure Rats

作　　者：赵乾[1] 黄晏[2] 张胜叶[1] 牛欢[1] 

机构地区：[1]兰州大学第一临床医学院,甘肃兰州730000 [2]兰州大学第一医院心内科,甘肃兰州730000

出　　处：《现代生物医学进展》2011年第8期1464-1466,1453,共4页Progress in Modern Biomedicine

摘　　要：目的:研究给予外源性Apelin-13治疗对血流动力学、血浆血管紧张素Ⅱ(Angiotensin-Ⅱ,AngⅡ)和肾上腺髓质素(Adrenomedulin,ADM)水平的影响。方法:将50只大鼠随机分为3组:心衰组(n=20)、治疗组(n=20)和正常对照组(n=10),阿霉素(ADR)腹腔注射建立大鼠心衰模型,治疗组给药外源性Apelin-13治疗,检测大鼠血浆AngⅡ和ADM水平(ELISA法)以及血流动力学的水平。结果:心衰组和治疗组血浆AngⅡ和ADM水平比正常对照组增高,但治疗组血浆AngⅡ和ADM水平比心衰组明显降低,治疗组左心室内压最大升降速度(LV±dp/dtmax)、左室最大收缩压(LVESP)比心衰组增高,左室舒张末期压(LVEDP)比心衰组低(P均<0.05)。结论:外源性apelin-13抑制体内的AngⅡ和ADM水平可能对ADR诱导的大鼠心衰有保护作用。Objective： To study the effect of exogenous apelin-13 on heart failure rats and detected the cardial function and the levels of the plasma AngⅡ（angiotensin-Ⅱ） and ADM（adrenomedulin）.Methods： A total of 50 rats were randomly divided into 3 groups including heart failure group（n=20）,therapy group（n=20） and normal group（n=10）.Rats heart failure models were induced by Adrenomedulin（ADR） injection intraperitoneally.When the models have created,the therapy groups were injected repectively with aplelin-13 for 10 days.Then we detect the cardial function by ELISA and the levels of the plasma Angiotensin-Ⅱ（AngⅡ） and Adrenomedulin（ADM）.Results： The plasma AngⅡ and ADM levels of the heart group and the therapy group were significant higher than the normal group,The therapy group＇s plasma AngⅡ and ADM levels were lower than the heart failure group＇s.In therapy group,the LV±dp/dtmax and LVESP were higher and LVEDP were lower than those in heart failure group（all P 0.05）.Conclusions： The exogenous apelin-13 can protect rat heart failure which induced by ADR probably through inhibiting the levels of plasma AngⅡand ADM.

关 键 词：APELIN-13 血管紧张素Ⅱ 肾上腺髓质素 

分 类 号：Q95-3[生物学—动物学] R54[医药卫生—心血管疾病]