阿莫西林对人HepG2细胞DNA有短暂损伤作用  

Amoxicillin shortly caused DNA damage in human HepG2 cells

作　　者：陈建明[1] 范冬薇[1] 王晶[1] 葛莉伟[1] 邱明[1] 

机构地区：[1]杭州师范大学生命与环境科学学院,杭州310036

出　　处：《中国抗生素杂志》2011年第5期370-373,共4页Chinese Journal of Antibiotics

基　　金：浙江省自然科学基金项目(Y2090698);浙江省钱江人才计划项目(2010R10062);生物科学国家特色专业建设项目

摘　　要：目的为了探讨阿莫西林对人HepG2细胞DNA是否有损伤作用。方法培养的人HepG2细胞经不同浓度(2、10、50和250μmol/L)阿莫西林处理1h或经50μmol/L阿莫西林处理不同时间(20、40、60、120和180min)后,运用单细胞凝胶电泳(SCGE)技术结合彗星图像软件(CASP)分析细胞尾部DNA百分率(tail DNA%)变化情况。结果不同浓度阿莫西林处理后结果显示,HepG2细胞尾部DNA百分率明显升高,至50μmol/L阿莫西林时达到最高值,各浓度处理组与不处理对照组相比差异皆有显著性(P<0.01);而同一浓度(50μmol/L)阿莫西林处理不同时间后结果显示,HepG2细胞尾部DNA百分率逐渐升高,至1h处理时间点时达到最高值,其后随着处理时间延长HepG2细胞尾部DNA百分率逐渐降低。结论阿莫西林对人HepG2细胞DNA有短暂损伤作用,阿莫西林诱发的HepG2细胞DNA损伤可能随时间延长逐渐被HepG2细胞本身修复除去。Objective To investigate whether amoxicillin could cause DNA damage in human HepG2 cells. Methods Exponentially grown HepG2 cells in 12-well plates were treated with different concentrations of amoxicillin （2, 10, 50 and 250μmol/L） for lh or treated with 50μmol/L amoxicillin for different time intervals （20, 40, 60, 120 and 180min）. After treatment, the single cell gel electrophoresis （SCGE） technique was used to analyze the tail DNA% of HepG2 cells combined with the comet image analyzing software （CASP） to see whether amoxicillin could cause DNA damage in HepG2 cells, since it is well known that the tail DNA% is directly proportional to the level of DNA strand breakage damage. Results It was found that the tail DNA% of HepG2 cells was obviously increased after the treatment with different concentrations of amoxicillin and it was reached to its highest level when treated with 50μmol/L amoxicillin, and statistically significant difference was observed comparing to the untreated control cells with each concentration of the treated cells （P〈0.01）. When HepG2 cells were treated with 50μmol/L amoxicillin for different time intervals, it was shown that the tail DNA% in HepG2 cells was gradually increased and reached to its highest level at lh treatment time point. However, after lh treatment point, the tail DNA% of HepG2 cells was then gradually decreased along with the duration of amoxicillin treatment. Conclusion It was concluded that amoxicillin could shortly cause DNA damage in HepG2 cells, but amoxicillin-induced HepG2 cell DNA damage probably could be gradually removed along with the time duration due to the repairing by the HepG2 cells per se.

关 键 词：阿莫西林 HEPG2细胞 单细胞凝胶电泳 DNA损伤 DNA修复 

分 类 号：R978.1[医药卫生—药品]