鹅源H9N2亚型禽流感病毒PB2和PB1基因序列分析  

Cloning and Sequence Analysis of PB2 and PB1 Genes of H9N2 Avian Influenza Virus Strain Isolated from Guangxi Goose

作　　者：付薇[1] 覃芳芸[1] 马琳[1] 徐贤坤[1] 黄胜斌[1] 易春华[2] 孙翔翔[2] 何奇松[2] 蒋家霞[2] 熊毅[1] 

机构地区：[1]广西动物疫病预防控制中心,广西南宁530001 [2]广西大学动物科学技术学院,广西南宁530005

出　　处：《动物医学进展》2011年第5期49-53,共5页Progress In Veterinary Medicine

基　　金：广西科技攻关项目(0719004-3G)

摘　　要：采用RT-PCR方法对1株鹅源H9N2亚型禽流感病毒(GS/GX/01/07)RNA聚合酶基因PB2和PB1分别进行了扩增,将其克隆到pMD18-T载体后进行序列测定与分析。结果表明,该株病毒的PB2、PB1开放阅读框(ORF)分别由2 280和2 277个碱基组成,分别编码759和758个氨基酸。经同源性和系统进化树分析,该毒株RNA聚合酶基因PB2和PB1与H9N2亚型欧亚谱系中的第2亚群代表株QA/HK/G1/97的核苷酸和氨基酸同源性在95%以上,亲缘关系较近。Full-length cDNAs of polymerase genes PB2 and PB1 of avian influenza virus strain GS/GX/01/06 were amplified by RT-PCR.The cDNAs were cloned into pMD18-T vectors and sequenced.Sequence analysis showed that open reading frames of the PB2 and PB1 cDNAs contain 2 280 nt and 2 277 nt encoding 759 and 758 amino acids,respectively.Nucleotide sequence and amino acid identity rates of the polymerase genes PB2 and PB1 were over 95% between avian influenza virus strain QA/HK/G1/97 that belong to the subtype H9N2 AIV,there were in closer relation between them.

关 键 词：禽流感病毒 H9N2亚型 聚合酶基因 序列分析 

分 类 号：S852.659.5[农业科学—基础兽医学]