洼地绵羊MHC-DRB1基因PCR-RFLP多态性分析  

Polymorphism Analysis of MHC-DRB1 Gene in Wadi Sheep by PCR-RFLP

作　　者：肖娜 任艳玲[2,3] 李敏 董文艳 沈志强[2,3] 

机构地区：[1]山东绿都生物科技有限公司,山东滨州256600 [2]山东省滨州畜牧兽医研究院,山东滨州256600 [3]山东省洼地绵羊繁育技术研究推广中心,山东滨州256600

出　　处：《动物医学进展》2011年第5期63-68,共6页Progress In Veterinary Medicine

基　　金：国家高技术研究发展计划(863计划)(2008AA10Z142);山东省自然科学基金(Y2007D12);山东省良种工程(2006GG22)

摘　　要：为了探讨洼地绵羊MHC-DRB1基因的多态性,采用套式PCR扩增82只洼地绵羊的MHC-DRB1基因第2外显子,其296 bp扩增产物经SacⅠ、HaeⅢ限制性内切酶酶切后进行RFLP多态性分析。结果表明,洼地绵羊的MHC-DRB1基因外显子2在SacⅠ、HaeⅢ的酶切位点存在多态性,测序发现,这些酶切位点分别受2、5个等位基因控制,由于MHC-DRB1为多碱基突变的基因位点,综合2种限制性内切酶的酶切结果,在洼地绵羊中共发现15种基因型,其中新的等位基因MHC-DRB1.D(225 bp/71 bp)仅在该洼地绵羊群体中发现。对MHC-DRB1的5个等位基因的核苷酸序列以及预测的氨基酸序列分别进行比对,发现5个等位基因间存在丰富的变异位点。To investigate the polymorphism of major histocompatibility complex ⅡDRB1（MHC-DRB1） in Wadi sheep,the second exon of this gene in 82 Wadi Sheep was amplified by nested PCR and the amplified products were characterized by RFLP technique using 2 restriction enzymes,ie,SacⅠandHaeⅢ.The results showed that the extensive polymorphisms of the exon2 of MHC-DRB1 were found at two loci digested with SacⅠandHaeⅢ,respectively,and these sites were controlled by two,five alleles,respectively.As some base mutations were found in MHC-DRB1locus,and according to the results in this paper,fifteen genetypes were found in Wadi sheep.The new allele MHC-DRB1.D was identified only in these Wadi sheep populations.The nucleotide and predicted amino acid sequence alignments of MHC-DRB1 alleles provided the evidence for considerable variation among the five alleles.

关 键 词：洼地绵羊 MHC-DRB1基因 聚合酶链反应和限制性片段长度多态性 多态性 

分 类 号：S852.165[农业科学—基础兽医学]