猪渗出性皮炎病原的分离鉴定  

Isolation and Identification of Pathogen from Exudative Epidermitis Swine

作　　者：张乐宜[1,2] 陈亚强[1,2] 蔡汝健[2] 郝永清[1] 宋长绪[2] 

机构地区：[1]内蒙古农业大学,内蒙古呼和浩特010018 [2]广东省农业科学院兽医研究所,广东广州510640

出　　处：《动物医学进展》2011年第5期124-127,共4页Progress In Veterinary Medicine

基　　金：广东省生猪科技创新体系岗位专家专项;广东省农业科技攻关项目(2003B21404;20052490100和2006B20301013);广东省自然科学基金(04002083);广东省自然基金重点项目(06105311)

摘　　要：从疑似渗出性皮炎的哺乳仔猪体内分离到一株细菌,经革兰染色镜检以及TH-16S细菌生化鉴定系统和葡萄球菌16 SrRNA、gap基因通用引物鉴定。结果表明,该菌株为葡萄球菌,gap基因的核苷酸同源性为99%。动物试验发现,试验小鼠出现躁动、被毛竖立、精神沉郁,18 h死亡,说明该菌株对小鼠具有致病力。The bacteriaisolated from a piglet affected with EE were identified by the methods of Gram-staining,TH-16s Bacteria Biochemical System and 16 s rRNA,gap gene sequencing.The results showed that this strain belongs to Staphylococcus hyicus,the nucleotide homology of gap gene is 99%.In the animal test,the mice presented dysphoriathe upright clothing hair,severe depression and died 18 h later.The results showed that this strain caused EE and had high pathogenicity.

关 键 词：猪渗出性皮炎 猪葡萄球菌 分离鉴定 表皮脱落毒素 致病性 

分 类 号：S853.55[农业科学—临床兽医学]