超声辅助萃取-高效液相色谱法测定牛奶中9种β-内酰胺抗生素残留  

Simultaneous determination of 9 β-lactam antibiotics in milk by HPLC with ultrasound-assisted extraction

作　　者：张尹[1] 毕莉[2] 徐兴志[1] 杨亚玲[1] 

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650224 [2]昆明理工大学理学院,云南昆明650093

出　　处：《云南大学学报（自然科学版）》2011年第3期332-335,339,共5页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(20767002)

摘　　要：建立了一种采用超声辅助萃取-高效液相色谱法检测牛奶中9种β-内酰胺抗生素残留的方法.牛奶样品经过乙腈沉淀蛋白,磷酸盐缓冲溶液为提取液,20 kHz超声处理5 min,离心处理,固相萃取小柱净化,采用Agilent TC C18(150 mm×Ф4.6 mm,5μm)色谱柱,选用磷酸盐-甲醇作流动相进行梯度洗脱,流速1.0 mL/min,220 nm检测,在22 min内分离检测.方法可用于牛奶中上述抗生素残留的同时检测.A method has been developed for the simultaneous determination of 9 β-lactam antibiotics residues in milk by high-performance liquid chromatography-ultrasonic assisted extraction（HPLC-UAE）.The milk samples were extracted with acetonitrile-phosphate buffer solution under 20kHz ultrasound for 5min,then centrifuged.With Agilent TC C18（150mm×4.6mm id,5μm） column,phosphate buffer-methanol as mobile phase,flow rate 1.0mL/min,220nm detection,9 β-lactam antibiotics were separated within 22min.The method could be used for simultaneous determination of β-lactam antibiotics in milk.

关 键 词：超声辅助萃取 高效液相色谱法 牛奶 Β-内酰胺抗生素 

分 类 号：O657.7[理学—分析化学]