元宝枫叶黄酮抑制脂多糖诱导的小胶质细胞激活的作用  

Attenuated LPS-induced activation of microglia by flavonoids from the leaves of Acer truncatum

作　　者：钟莲梅[1] 宗一[2] 戴纪男[2] 杨萍[2] 张伟[1] 詹东[2] 陆地[2] 孙俊[2] 

机构地区：[1]昆明医学院第一附属医院神经内科,云南昆明650011 [2]昆明医学院人体解剖学教研室,云南昆明650500

出　　处：《云南大学学报（自然科学版）》2011年第3期345-349,359,F0003,共7页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然基金资助项目(30860336);云南省科技厅-昆明医学院联合资助项目(2007C0005R2008CD053);云南省中青年学术和技术带头人后备人才培养项目(2009CI033)

摘　　要：探讨枫叶黄酮对脂多糖(Lipopolysaccharide,LPS)诱导的小胶质细胞株BV-2细胞炎性因子释放的抑制作用.用LPS刺激BV-2细胞构建炎症模型,采用免疫荧光双标和RT-PCR方法检测不同浓度枫叶黄酮(5,10,15μmol/L)对细胞炎性蛋白酶诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)和环氧合酶-2(cyclooxygenase-2,COX-2)、细胞炎性因子肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白介素-1β(interleukin-1β,IL-1β)、炎性信号蛋白核因子-κB(nuclear factor-κB,NF-κB)蛋白与mRNA的表达变化.结果发现:不同浓度的枫叶黄酮在翻译水平和转录水平上明显抑制了LPS诱导的细胞炎性蛋白酶iNOS和COX-2,细胞炎性因子TNF-α和IL-1β与炎性信号蛋白NF-κB的上调.上述结果表明枫叶黄酮可通过调控LPS诱导的小胶质细胞株BV-2细胞炎性因子释放从而抑制小胶质细胞激活,发挥抗神经炎症的作用.To investigate suppressing effects of flavonoids from the leaves of Acer truncatum in the activation od BV-2 microglia,cells were treated with flavonoid（5,10,15μmol/L） prior to LPS exposure,then the effects on the mRNA and protein levels of pro-inflammatory enzymes,inducible nitric oxide synthase（iNOS）,cyclooxygenase-2（COX-2）,pro-inflammatory cytokines,tumor necrosis factor-α（TNF-α）,interleukin-1β（IL-1β） and inflammatory signaling proteins nuclear factor-κB（NF-κB） were analysed by RT-PCR and double-immunofluorescence labeling assay.The results indicate that flavonoid has an effect on the expression of iNOS,COX-2,TNF-α,IL-1β and NF-κB through transcriptional and translational inhibition.Therefore we think the flavonoid can restrain microglial cells activity by means of regulation pro-inflammatory mediators of BV-2 cells that induced by LPS,so it can exert anti-neuroinflammatory actions.

关 键 词：枫叶黄酮 脂多糖 炎性因子 BV-2细胞 

分 类 号：Q591.8[生物学—生物化学]