柔嫩艾美耳球虫敏感株与耐药株成熟卵囊中葡萄糖含量的检测  

Detection of the Glucose Level in Mature Oocyst of Drug Sensitive or Resistant Eimeria tenella Strains

作　　者：马立农[1] 陈杖榴[2] 余亮彬[3] 

机构地区：[1]深圳职业技术学院应用化学与生物技术学院,广东深圳518055 [2]华南农业大学兽医药理研究室,广州510642 [3]黄冈职业技术学院生物化工学院,湖北黄冈438002

出　　处：《湖北农业科学》2011年第8期1647-1649,共3页Hubei Agricultural Sciences

摘　　要：建立了一种采用比色测定中性糖的硫酸-酚法测定柔嫩艾美耳球虫成熟卵囊中葡萄糖含量的方法,并对柔嫩艾美耳球虫(Eimeria.tenella)的敏感株和各种耐药株共9个球虫株的成熟卵囊中葡萄糖含量进行了测定。结果表明,敏感株成熟卵囊中的葡萄糖含量较低,而耐药株成熟卵囊中葡萄糖含量则相对较高,二者比较呈现极显著相关性(P<0.01)。由此可见,柔嫩艾美耳球虫对抗球虫药物的敏感性可能与成熟卵囊中葡萄糖含量有关,通过对成熟卵囊中葡萄糖含量的测定可作为判别柔嫩艾美耳球虫是否耐药的指标之一。A method employing the oil of vitriol and phenol testing neutral sugars to detect the level of glucose in mature oocyst of Eimeria tenella was established.The level of glucose in mature oocyst of 9 different stains in E.tenella（including sensitive stain and drug-resistant stains）were detected.The results were as follows,the level of glucose in mature oocyst of sensitive strain was low;the level of glucose in mature oocyst of the drug-resistant strains was high（P0.01,showed significant correlation）.Thus,the stains of E.tenella with different drug sensitivity were related with the level of glucose in mature oocyst.The level of glucose in mature oocyst could be used as one of the indicators to anticoccidial drug sensitivity of E.tenella.

关 键 词：柔嫩艾美耳球虫 敏感株 耐药株 葡萄糖含量 

分 类 号：S852.723[农业科学—基础兽医学]