TMB-8抑制神经递质引起的单个脑细胞内游离钙的升高(英文)  

Inhibitory effects of 8- (N, N-diethylamino) -n-octyl-3,4,5trimethoxybenzoate (TMB-8) on intracellular Ca^(2+) elevated by neurotransmitters in brain cells

作　　者：张孝清[1] 王斌[1] 张民英[1] 肖继皋[1] 

机构地区：[1]南京医科大学药理教研室,南京中国210029

出　　处：《中国药理学报》1999年第10期893-896,共4页Acta Pharmacologica Sinica

摘　　要：目的:研究TMB-8对神经递质引起的单个脑细胞内游离钙升高的作用。方法:应用AR-CM-MIC阳离子测定系统测定游离大鼠单个脑细胞内钙离子浓度。结果:当细胞外液Ca^(2+)浓度为1.3mmol·L^(-1)时,TMB-8 30μmol·L^(-1)能降低谷氨酸,组织胺,5-羟色胺引起的脑[Ca^(2+)]_i浓度的升高。而当细胞外液无钙时,TMB-8能降低细胞内静息[Ca^(2+)]_i;TMB-8 10μmol·L^(-1)则几乎完全抑制了组织胺和5-羟色胺引起的脑[Ca^(2+)]_i升高作用。结论:TMB-8能降低谷氨酸,组织胺,5-羟色胺引起的脑[Ca^(2+)]_i升高。AIM: To study the effects of TMB-8 on [Ca2+]i elevation induced by neurotransmitters in dissociated brain cells. METHODS: The brain cell suspension was made using a gentle trituration for 1 min with a polished pipette. The changes of [ Ca2 + ]; were detected by the fluorescent indicator, Fura 2-AM. RESULTS:In the presence of extracellular Ca2+ 1.3 mmol · L-1, sodium glutamate ( Glu ), histamine (His), and serotonin (5-HT) markedly increased the [Ca2+ ]i which were reduced by TMB-8 30 μmol·L-1. TMB-8 3 μmol·L-1 produced inhibitory effects on the increase of [Ca2+ ]i by His and 5-HT in a Ca2 + -free Hanks' solution. The increase of [ Ca2 + ]; by His and 5-HT was reduced to control level by TMB-8 10μmol · L-1. CONCLUSION: TMB-8 inhibited the [Ca2 + ]i elevation induced by Glu, 5-HT, and His in brain cells.

关 键 词：脑细胞 游离钙 尼莫地平 谷氨酸钠 血清素 药理 

分 类 号：R972[医药卫生—药品]