丁基苯酞对牛脑微动脉及胸主动脉内皮血管活性物质生成的影响(英文)  

Effects of 3-n-butylphthalide on production of vasoactive substances by cerebral and aortic endothelial cells

作　　者：徐皓亮[1] 冯亦璞[2] 

机构地区：[1]中国医学科学院,北京中国100050 [2]中国协和医科大学药物研究所,北京中国100050

出　　处：《中国药理学报》1999年第10期929-933,共5页Acta Pharmacologica Sinica

基　　金：Project supported by the State Science and Technology Commission grant (№ 94-ZD-01) and by the National Natural Science Foundation of China(№ 29790122).

摘　　要：目的:观察消旋、左旋、右旋丁基苯酞(dl,l,d-NBP)对体外培养的脑血管内皮细胞(BCEC)及主动脉内皮细胞(BAEC)合成一氧化氮(NO),前列环素(epoprostenol,Epo),内皮素-1(ET-1)的影响。方法:自牛大脑灰质及胸主动脉分离培养内皮细胞,并用分光光度法和放免法测定细胞培养液中NO,Epo,ET-1的含量。结果:BCEC及BAEC可持续分泌NO,Epo,ET-1,dl,l-NBP可以剂量依赖性地明显促进BAEC及BCEC中NO的合成,不同浓度的d-NBP均能增加BAEC中NO的产生,而对BCEC无明显作用。低浓度的dl,l-NBP(0.1-10μmol·L^(-1))即可显著升高BCEC中Epo的产生,而对BAEC无明显影响。另外,dl,l,d-NBP对TNFα诱导的BAEC中ET-1增加均无显著影响。结论:1)dl-和l-NBP均可显著增加BAEC及BCEC中NO的释放。2)l-NBP可升高BCEC和BAEC中Epo的释放,且对BCEC具有选择性。dl-NBP选择性增加BCEC中Epo的释放。AIM: The effects of dl-3-n-butylphthalide ( dl-NBP), l-3-n-butylphthalide (l-NBP), and d-3-n-butylphthalide ( d-NBP) on the production of nitric oxide (NO), epoprostenol (Epo) and endothelin-1 (ET-1) were investigated in cerebrovascular and aortic endothelium in culture. METHODS: Bovine cerebral endothelial cells (BCEC) and bovine aortic endothelial cells (BAEC) were cultured in Medium 199 in vitro. After incubation with dl-, l-, and d-NBP for 24 h, the release of NO, Epo, and ET-l were analyzed by using spectrometry assay and radioimmunoassay (RIA) respectively. RESULTS: Low concentrations of dl-and l-NBP (0.1-10 μmol·L-1) enhanced nitriite and 6-ketoprostaglandin F1α (6-ketoPGF1α) production in both BAEC and BCEC after a 24-h incubation, and l-NBP has a potent effect on promoting Epo production in BCEC. The production of ET-1 secreted by BCEC and BAEC was increased after TNFαstimulation, this enhancement was not blunted by the simultaneous addition of dl-, l-, and d-NBP. CONCLUSION: 1) dl-NBP and l-NBP increase NO production in both BCEC and BAEC. 2) l-NBP increases more Epo production in BCEC than that in BAEC, and dl-NBP has selective effect on increasing Epo production inBCEC.

关 键 词：丁基苯酞 内皮素-1 大脑皮质 主动脉 药理 

分 类 号：R971.9[医药卫生—药品]