FD耐热逆转录酶介导的逆转录 nestedPCR检测丙型肝炎病毒  

Detection for HCV with FD thermostable reverse transcriptase mediated RT nested PCR

作　　者：郑佐华[1,2] 张丽萍[1,2] 赵炜[1,2] 张炜 陈燃[1,2] 毛裕民[1,2] 

机构地区：[1]复旦大学遗传所,上海200433 [2]复旦大学遗传工程国家重点实验室

出　　处：《中华微生物学和免疫学杂志》1999年第5期445-447,共3页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金;上海市自然科学基金

摘　　要：目的　为检测丙型肝炎病毒，建立 Ｆ Ｄ 耐热逆转录酶介导的逆转录ｎｅｓｔｅｄ Ｐ Ｃ Ｒ 系统。方法　用该系统对１０８ 例可疑患者的血液进行检测，并与 Ｅ Ｌ Ｉ Ｓ Ａ 法的结果进行比较。结果　实验表明， Ｆ Ｄ 耐热逆转录酶介导的逆转录ｎｅｓｔｅｄ Ｐ Ｃ Ｒ 比 Ｅ Ｌ Ｉ Ｓ Ａ 检出了更高的阳性率。结论　该系统是一种灵敏、有效、特异的 Ｈ Ｃ Ｖ 检测方法。Detection for HCV with FDthermostable reverse transcriptase mediated RTnested PCR$$$$ ZHENG Zuohua, ZHANG Liping, ZHAO Wei, et al. Institute of Genetics, State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433 【 Abstract 】 Objective In order to detect HCV in human blood, a FDthermostable reverse transcriptase mediated RTnested PCR technique was developed. Methods For comparison, we used this method and conventional ELISA to detect the infection of HCV in 108 patients. Results The results showed higher positive rate in FDTRT mediated RTnested PCR. Conclusion The results suggest that it is a more sensitive, effective and specific way for detecting HCV infection. 【 Subject words 】 FDthermostable reverse transcriptase Polymerase chain reaction Hepatitis C virus

关 键 词：FD 耐热逆转录酶 nestedPCR 丙型肝炎病毒 

分 类 号：R373.21[医药卫生—病原生物学]