1株鸭源新城疫病毒的分离鉴定及生物学特性研究  

Identificationand Biological Characterization of a Newcastle Disease Virus from Duck

作　　者：刘大伟[1] 焦培荣[1] 黎先伟[1] 何琴[1] 高诗敏[1] 漆丹丹[1] 陈德成[1] 任涛[1] 

机构地区：[1]华南农业大学兽医学院,农业部动物疫病防控重点开放实验室,广东省动物源性人兽共患病预防与控制重点实验室,广东广州510642

出　　处：《中国畜牧兽医》2011年第5期143-145,共3页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31072319);公益性行业(农业)科研专项经费(200803020-08)

摘　　要：从广东地区发病鸭中分离到1株新城疫病毒,血凝试验、血凝抑制试验结果表明此分离株具有血凝活性,且这种血凝性可被NDV标准阳性血清抑制,而不能被AIV-H5、AIV-H7、AIV-H9、EDSV阳性血清抑制;用针对NDVF基因特异性鉴定引物对该分离株进行聚合酶链反应扩增,可扩增出相应的目的片段。由此,初步鉴定该分离株为鸭源新城疫病毒,并命名为NDV/DUCK/SD/2006。另外,通过毒力测定,该毒株的MDT、IVPI分别为51.75 h、2.44,判定为强毒力株。A Newcastle disease virus（NDV） was isolated from duck in Guangdong.It was identified with hemagglutination,hemagglutination inhibition.These results showed the isolate had hemagglutination and was inhibited by the standard antibody of AIV-H5,AIV-H7,AIV-H9 and EDSV.It was amplified by RT-PCR and sequenced,which was used by the specific identification primers of NDV F gene.Thus,the isolate was identified as a duck NDV,named NDV/DUCK/SD/2006.In this research,the isolate were conducted virulence determination.Results showed that,the MDT,and IVPI of the isolate were 51.75 h,2.44 respectively,suggesting it was a velogenic strain.

关 键 词：新城疫病毒 鸭 广东 生物学特性 

分 类 号：S858.32[农业科学—临床兽医学]