徒手克隆工具自制及改进  

Production and Improvement of Handmade Somatic Cell Cloning Tools

作　　者：黄章虎[1] 任艳萍[1] 谭世俭[1] 

机构地区：[1]广西大学动物繁殖研究所,广西南宁530004

出　　处：《中国畜牧兽医》2011年第5期237-240,共4页China Animal Husbandry & Veterinary Medicine

基　　金：广西科学基金(桂科自0832039);广西研究生教育创新计划资助项目

摘　　要：徒手克隆技术(handmade somatic cell cloning,HMC)解决了由于昂贵设备对核移植技术应用的制约问题。通过徒手克隆刀的自制,不但进一步节省了成本,且更能符合使用者的习惯,提高总的切割效率。试验结果表明,利用自制克隆刀进行半卵法去核,可以达到每小时切割496枚卵母细胞的切割速度和88.14%的存活率;自制聚集针具有制作简单,可以打磨、效果好等特点,可以进一步简化和降低徒手克隆技术的成本,提高克隆效率。Handmade somatic cell cloning（HMC） technology resolves the huge difficulty for the application of somatic cell nuclear transfer technology in the actual production which is the high equipment costs.It is not only save more costs by using self-made micro-knife,but also could fit user＇s customary well,so it improves cutting efficiency.The results showed that the speed of enucleation was 496 oocytes per hour by cutting oocytes used self-made micro-knife and the recovered rate was 88.14%.Moreover,the feature of Well of Well needle which was easy to produce and could be polished.It was play a important role in cost saving and improving efficiency of HMC technology.

关 键 词：徒手克隆 自制克隆刀 自制聚集针 徒手切割方法 

分 类 号：Q785[生物学—分子生物学]