高效液相色谱法测定烟火药剂中的苦味酸  

Determination of Picric Acid in Pyrotechnic Composition by High Performance Liquid Chromatography

作　　者：江放明[1,2] 戴斐[3] 谭爱喜[1] 黄志强[1] 张莹[1] 周方钦[3] 

机构地区：[1]湖南出入境检验检疫局 [2]中南大学化学化工学院 [3]湘潭大学化学学院

出　　处：《光谱实验室》2011年第3期1142-1145,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：2008年国家公益性科研项目(10-70)

摘　　要：建立了烟火药剂中苦味酸的高效液相色谱检测方法。采用Zorbax Eclipse XDB-C18色谱柱(4.6mm×250mm,5μm)分离,V(甲醇)∶V(0.1%冰醋酸水溶液)=55∶45,等梯度洗脱,流速0.8mL/min;测定温度为40℃;采用紫外检测器检测,检测波长为350nm。苦味酸的质量浓度在0.5—20mg/L时与色谱峰面积之间线性关系良好(r=0.9998);加标回收率为85.6%—92.4%,相对标准偏差(RSD)为2.1%—6.5%。该方法简便快速,结果准确,重现性好,可作为检测烟火药剂中苦味酸的一个有效方法。A method for the determination of picric acid in pyrotechnic composition by high performance liquid chromatography was developed.The separation were performed on a Zorbax Eclipse XDB-C18 column（4.6mm×250mm,5μm）with methanol-0.1% acetic acid solution（volume ratio of 55∶45） as the mobile phase at the flow rate of 0.8mL/min and the temperature of 40℃.The detection wavelength was 350nm for ultraviolet detector.There were good linear relationships between the mass concentration and the peak areas of picric acid in the range of 0.5—20mg/L（r=0.9998）.The recoveries were 85.6%—92.4% with the relative standard deviations of 2.1%—6.5%.The method is quick,simple with accurate results and good repeatability,and can be used for the determination of picric acid in pyrotechnic composition.

关 键 词：高效液相色谱法 苦味酸 烟火药剂 

分 类 号：O657.72[理学—分析化学]