荧光分析法测定岩黄芪根中的总黄酮含量  

Determination of Total Flavonoids in Polybotrys Roots by Fluorescent Analysis

作　　者：巴吐尔.买买提明 李月红[2] 海力茜.陶尔大洪 旷南岳[3] 

机构地区：[1]新疆医科大学分析测试中心,乌鲁木齐市830054 [2]新疆医科大学第一附属医院中医科,乌鲁木齐市830054 [3]新疆医科大学药学院药物分析教研室,乌鲁木齐市新医路8号830054

出　　处：《光谱实验室》2011年第3期1302-1305,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：新疆维吾尔自治区少数民族科技骨干特培专项基金(No.2003-4)

摘　　要：采用索氏提取法,超声提取法,回流提取法分别提取多序岩黄芪、天山岩黄芪根中总黄酮,并用荧光分析法进行含量测定。结果测得回流提取法提取的多序岩黄芪、天山岩黄芪根中总黄酮含量分别为12.005%,8.647%;索氏提取法提取的总黄酮含量分别为9.744%,7.348%;超声提取法提取的总黄酮含量分别为7.688%,5.840%。最后得出结论回流提取法是提取多序岩黄芪根中总黄酮的最佳方法。The determination of the contents of total flavonoids in Hedysarum polybotry and Hedysarum Semenovii Regelet Herd roots were respectively exacted by Soxhlet extraction,ultrasonic method and refluxing extraction were investigated by fluorescent analysis.The contents of total flavonoids in Hedysarum polybotry and Hedysarum Semenovii Regelet Herd roots for three extraction methods were follow：refluxing extraction were12.005% and 8.647%,then Soxhlet extraction were 9.744% and 7.348%,while ultrasonic extraction were 7.688% and 5.840%,respectively.The refluxing extraction is optimal method for determination of total flavonoids in Hedysarum polybotry roots.

关 键 词：荧光分析 多序岩黄芪根 天山岩黄芪根 总黄酮含量 

分 类 号：O657.32[理学—分析化学]