DFMG拮抗LPC诱导主动脉内皮细胞凋亡  

Antagonistic effects of 7-difluoromethoxy-5,4'-dimethoxygenistein on human aorta endothelial cell injury induced by lysophosphatidylcholine

作　　者：李程[1] 刘飞[1] 全梅芳[1] 曹建国[1] 符晓华[1] 

机构地区：[1]湖南师范大学医学院,湖南长沙410013

出　　处：《中国现代医学杂志》2011年第11期1297-1301,1306,共6页China Journal of Modern Medicine

基　　金：国家自然科学基金资助项目(No:30971270);湖南省自然科学基金重点资助项目(No:07JJ3047)

摘　　要：目的研究7-二氟甲氧基-5、4’-二甲氧基金雀异黄素(7-difluoromethoxy-5,4’-dimethoxy-genistein,DFMG)对溶血性磷脂酰胆碱(lysophosphatidylcholine,LPC)诱导人主动脉内皮细胞(human aorta en-dothelial cells,HAEC)凋亡的影响。方法体外培养HAEC。Annexin V/PI染色流式细胞术(FCM)分析细胞凋亡率。ELISA法测定Caspase-3、8和9活性。Western blotting分析细胞色素c和死亡受体-4、-5表达。结果 LPC(10μmol/L)处理HAEC 24 h,细胞凋亡率为(31.6%±4.1%)(P<0.001)。DFMG预孵育降低LPC诱导HAEC细胞凋亡率,Caspase-3、-8和-9活化及细胞色素C和死亡受体-5表达(P<0.05)。结论 DFMG拮抗LPC诱导HAEC细胞凋亡作用与阻断LPC激活死亡受体和线粒体凋亡途径有关。【Objective】To investigate the effects of 7-difluoromethoxy-5,4＇-dimethoxygenistein（DFMG） on human aorta endothelial cell（HAEC） injury induced by lysophosphatidylcholine（LPC）.【Methods】HAEC were cultured in vitro.The apoptotic rate was detected by flow cytometry using Annexin V/PI staining.The enzymatic activity of caspase-3,-8 and-9 were determined by ELISA.Cytochrome C and the death re-porter-4（DR4）,-5（DR5） expressions of HAEC were determined by Western blot.【Results】Treatment with 10 μmol/L LPC for 24 h,the apoptotic rate of HAEC increased to（31.6 ± 4.1）%（P 0.001）.The pretreatment with DFMG significantly decreased induction of apoptotic cell death,the activation of caspase-3,-8 and-9 and the expression of Cytochrome C and DR5 by LPC treatment.【Conclusion】Antagonistic effects of DFMG on HAEC injury induced by LPC is associated with the blockage of LPC-induced death reporter and mito-chondrial pathways.

关 键 词：7-二氟甲氧基-5、4’-二甲氧基金雀异黄素 主动脉内皮细胞 溶血性磷脂酰胆碱 细胞凋亡 死亡受体-5 

分 类 号：R543.5[医药卫生—心血管疾病] R813.5[医药卫生—内科学]