RP-HPLC法测定复方鱼腥草片中(R,S)-告依春的含量  

RP-HPLC determination of (R,S)-epigoitrin in compound Yuxingcao tablets

作　　者：索银科 刘学东[2] 王永平[3] 

机构地区：[1]宝鸡市药品检验所,宝鸡721000 [2]宝钛集团有限公司职工医院,宝鸡721014 [3]中铁一局集团华县中心医院,华县714100

出　　处：《药物分析杂志》2011年第5期900-902,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的：建立反相高效液相色谱法测定复方鱼腥草片中（R,S）-告依春的含量。方法：采用C18色谱柱,以0.05%磷酸溶液-乙腈（93∶7）为流动相,流速0.8 mL·mL^-1,检测波长245 nm,柱温30℃。结果：（R,S）-告依春在0.028~0.280 mg·mL^-1范围内具有良好的线性关系,r=0.9995;平均回收率为99.7%。结论：该方法可作为复方鱼腥草片的质量控制方法。Objective：To establish an RP-HPLC method for determination of（R,S）-epigoitrin in compound Yuxingcao tablets.Methods：A C18 column was adopted with 0.05% phosphoric acid-acetonitrile（93∶7）as the mobile phase at a flow rate of 0.8 mL·min^-1,the detection wavelength was 245 nm and the column temperature was 30 ℃.Results：The calibration curve was linear in the range of 0.028-0.280 mg·mL^-1（r=0.9995）.The average recovery was 99.7%.Conclusion：The method can be used for determination of（R,S）-epigoitrin in compound Yuxingcao tablets.

关 键 词：高效液相色谱法 复方鱼腥草片 (R S)-告依春 

分 类 号：R917[医药卫生—药物分析学]