DNA修饰电极上盐酸异丙嗪电化学行为及其电化学动力学性质  

Electrochemical behavior and electrochemical kinetics of promethazine hydrochloride at a DNA modified carbon paste electrode

作　　者：宋华[1] 顾玲[1] 

机构地区：[1]教育部轻化工助剂化学与技术重点实验室,陕西科技大学,西安710021

出　　处：《药物分析杂志》2011年第5期983-986,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:研究盐酸异丙嗪(promethazine hydrochloride)在DNA修饰碳糊电极(DNA/CPE)上的电化学行为及电化学动力学性质。方法:用循环伏安法(CV)、线性扫描伏安法(LSV)、计时库仑法(CC)和计时电流法(CA)等多种电化学方法进行研究。结果:在pH=6.8的C-L缓冲溶液中,1.0×10-4mol.L-1的盐酸异丙嗪在DNA/CPE上氧化峰电位为0.633 V,比在碳糊电极(carbon paste electrode,CPE)上的峰电位正移了30 mV,峰电流显著降低。测得盐酸异丙嗪在DNA/CPE上电极反应动力学参数:电荷转移系数α=0.80,扩散系数D=8.936×10-6cm2.s-1,电极反应速率常数kf=3.828×10-2cm2.s-1,而在CPE上α=0.86,扩散系数D=1.099×10-5cm2.s-1。结论:盐酸异丙嗪与DNA二者以嵌插作用结合,形成了一种非电活性物质。Objective：To investigate the electrochemical behavior of promethazine hydrochloride and its electrochemical kinetics at a DNA modified carbon paste electrode（DNA/CPE）.Methodes：Cyclic voltammetry（CV）,linear sweap voltammetry（LSV）,chronocoulometry（CC）and chronoamperometry（CA）were used in the present research.Results：In a pH 6.8 C-L buffer solution,the electrochemical behavior of 1.0×10^-4 mol·L^-1 promethazine hydrochloride was an irreversible electrochemical oxidation process on DNA/CPE and that oxidation peak potential was 0.633 V,in contrast to at the carbon paste electrode（CPE）,the oxidation peak potential（Ep）shifted positively and the peak current（ip）declined.Conclusion：By the determination of electrochemical parameters by the DNA/CPE and CPE,it is deduced that a non-eletroactive super-molecular compound is generated between DNA and promethazine hydrochloride.

关 键 词：盐酸异丙嗪 DNA修饰电极 电化学行为 电化学动力学 

分 类 号：R917[医药卫生—药物分析学]