驼背鲈外周血细胞的形态学研究  

Morphological study of the peripheral blood cells of highfin grouper Cromileptes altivelis

作　　者：王静香[1,2] 李加儿[1] 区又君[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《海洋科学》2011年第4期77-82,88,共7页Marine Sciences

基　　金：广东省科技计划项目(C20817);广东省重大科技兴海(渔)项目(A200501A04)

摘　　要：利用Wright’s和Giemsa双重染色法和改良的血涂片染色法两种方式,通过光镜对驼背鲈(Cromileptes altivelis)的血涂片进行了观察。结果表明:驼背鲈的血涂片中可区分出6种细胞——红细胞、嗜中性粒细胞、嗜酸性粒细胞、单核细胞、淋巴细胞、血栓细胞,偶尔可见到分裂的红细胞、分裂的血栓细胞、核影红细胞和聚集的白细胞。此外,统计了红细胞数1.7462×106个/mm3,白细胞数4.7817×103个/mm3,并对白细胞进行了分类计数:淋巴细胞37.26%、血栓细胞30.82%、嗜中性粒细胞23.57%、单核细胞8.35%、嗜酸性粒细胞极少见。描述了以上各细胞的显微结构。单核细胞的体积最大,小淋巴细胞的体积最小;淋巴细胞的数量最多,嗜酸性粒细胞数量最少。Using the Wright-Giemsa double staining method and improved blood smears of two staining methods, the peripheral blood cells of the Cromileptes altivelis were studied by light microscopy. Six major types of cells were recognized： erythrocyte, neutrophil, eosinophilic granuloeyte, monocyte, lymphocyte, and thrombocyte. In blood smears, erythrocyte division, divided thrombosis cells, the shadow of erythrocyte, and leukocyte aggregation could be observed. The quantities of erythrocytes and leukocyte were 1.7462×10^6/mm^3 and 4.7817×10^3/mm×3, respectively. The percentages of lymphocytes, thrombocyte, neutrophil and monocytes were 37.26%, 30.82%, 23.57% and 8.35% respectively, while eosinophilic granuloeyte was few. The microstructure of the above cells was described. The size of small lymphocyte was the smallest while that of the monocyte was the largest; the number of neutrophil was the least while that of lymphocytes was the most in the blood cells.

关 键 词：驼背鲈(Cromileptes altivelis) 血细胞 形态学 

分 类 号：Q959.483[生物学—动物学]