转化奎宁酮为(R)-3-奎宁醇菌株的筛选及转化条件优化  

Isolation of a Rhodotourula mucilaginosa strain capable of transforming quinuclidinone into(R)-3-quinuclidinol and optimization of the transforming reaction conditions

作　　者：邱健[1] 张霞[1] 马宏[1] 张铎[2] 宋水山[1] 

机构地区：[1]河北省科学院生物研究所,河北石家庄050051 [2]河北化工医药职业技术学院,河北石家庄050051

出　　处：《微生物学通报》2011年第5期736-742,共7页Microbiology China

基　　金：河北省科学院青年创新基金项目(No.10324);河北省科技支撑计划项目(No.09205519D)

摘　　要：(R)-3-奎宁醇是一种用于合成各类药物的重要手性砌块,以奎宁酮盐酸盐为唯一碳源,筛选得到一株能够将奎宁酮不对称还原为(R)-3-奎宁醇的菌株X15。常规生理生化鉴定和18S rDNA序列分析表明,菌株X15属于粘红酵母菌Rhodotorula mucilaginosa,定名为R.mucilaginosa X15。结果显示,菌株X15具有酮基还原能力和辅酶再生能力,在100 mL反应体系中可将奎宁酮还原为(R)-3-奎宁醇,转化率90%,ee值为88%。（R）-3-Quinuclidinol is an important chiral building block for the synthesis of a variety of pharmaceutical.A strain which is able to transforming quinuclidinone into reductase（R）-3-Quinuclid inol was isolated from soil by using quinuclidinone as sole carbon sourse in the screening medium.The physiological and bio-chemical identification and 18S DNA sequense analysis revealed that this strain belongs to Rhodotorula mucilaginosa and was named as R.mucilaginosa X15.The present results in-dicated that the optimal conditions for the transforming reaction with resting cells of R.mucilaginosa X15 were 3-quinuclidinone 10 g/L,pH 7.5,30 °C and 72 h reaction time.（R）-3-Quinuclidinol is ob-tained with an yeild of 90% and with an optical purity of 88% enantiometric excess（ee） from a 100 mL reaction mixture.

关 键 词：奎宁酮酮基还原酶 粘红酵母 对称还原 (R)-3-奎宁醇 

分 类 号：TQ463[化学工程—制药化工]