TP-M13-SSR技术在梨遗传多样性研究中的应用  

TP-M13-SSR technique and its applications in the analysis of genetic diversity for pear germplasm resources

作　　者：高源[1] 田路明[1] 刘凤之[1] 曹玉芬[1] 

机构地区：[1]中国农业科学院果树研究所/农业部果树种质资源利用重点开放实验室,辽宁兴城125100

出　　处：《果树学报》2011年第3期394-399,共6页Journal of Fruit Science

基　　金：中央级公益性科研院所基本科研业务费专项(2060302-6-1);国家梨产业技术体系建设专项(nycytx-29-2)

摘　　要：利用在SSR扩增产物检测过程中的一种基于荧光测序技术的高通量低成本分析技术体系TP-M13-SSR(simple sequence repeat with tailed primer M13)对5个种25份梨种质资源材料进行了SSR标记的遗传多样性研究。利用NH013a和NH007b 2对引物即可区分所有供试品种,5对引物的区分率平均为76%。25份材料在5个SSR位点的遗传多样性、多态性信息含量和位点杂合度的变化分别为0.656 0～0.834 60、0.639 8～0.814 9和0.434 8～1.000 0。新疆梨的遗传多样性水平和多态性信息含量最低,平均值分别为0.716 0和0.681 2;秋子梨最高,平均值分别为0.772 0和0.737 9。TP-M13-SSR方法具有经济、灵敏、高效等优点,在梨遗传多样性研究中应用效果好。An economical method of simple sequence repeat with tailed primer M13（TP-M13-SSR） was used in genetic diversity analysis for 25 cultivars of 5 species,in which fluorescent labeling of SSR-PCR products was used.Two pairs of primers NH013a and NH007b could distinguish all the pear cultivars,the identified rate of 5 pairs of primers was 76% on average.The ranges of gene diversity,PIC and locus heterozygosity on 5 SSR loci were 0.656 0 to 0.834 60,0.639 8 to 0.814 9 and 0.434 8 to 1.000 0 respectively.The gene diversity and PIC of Pyrus sinkiangensis which were 0.716 0 and 0.681 2 on average respectively were the lowest,but those of P.ussuriensis which were 0.772 0 and 0.737 9 on average respectively were the highest.The method has the advantages of high-throughput,sensitiveness,cost-effectiveness and high accuracy.And it has been used in studies on genetic diversity analysis of pear successfully.

关 键 词：梨 TP-M13-SSR 遗传多样性 

分 类 号：S661.2[农业科学—果树学]