溶剂/流速双梯度-HPLC整体柱分析三七中药配方颗粒中4种皂苷成分  

Organic Solvent/Flow Double-Gradient HPLC Determination of Four Saponins in Sanqi Chinese Medicinal Granules Using Monolithic Column

作　　者：李宁[1] 潘晓玲[1] 黄光亮[1] 高崇凯[1] 

机构地区：[1]广东药学院,广州510006

出　　处：《化学学报》2011年第8期981-986,共6页Acta Chimica Sinica

摘　　要：建立一种新的溶剂/流速双梯度-整体柱高效液相色谱法快速测定三七中药配方颗粒中4种皂苷成分.通过乙腈浓度、流速与被测物的lg k线性相关性,预测被测组分三七皂苷R1,人参皂苷Rg1与人参皂苷Re的适宜k值,以测得的分离度为优化指标,建立分离R1,Rg1,Re的最佳分离模式1和2;在此基础上,通过考察乙腈浓度和流速随时间的变化率对分离人参皂苷Rb1的影响,以Rb1与相邻杂质的分离度为优化指标,得到分离R1,Rg1,Re,Rb1的两种最佳模式.应用色谱优化函数(COF)作为综合指标评价,结果显示模式2优化效果更好.采用模式2的色谱条件,20 min内完成对三七配方颗粒中4种皂苷成分的质量分析,结果表明该方法准确可行、快速.A combined organic solvent/flow double-gradient mode in HPLC using a monolithic column is established as a new approach to determination of four saponins in Sanqi Chinese Medicinal Granules.The correlation between content of acetonitrile and lg k,flow and lg k,by which suitable k values of notoginse-nosid R1,ginsenoside Rg1 and Re were calculated.The resolution of test analytes were used as a criterion,the good separation mode 1 and 2 of R1,Rg1 and Re were obtained.In developing the separation ginsenoside Rb1 the effect on the changes of acetonitrile content and flow with time,the resolution of the critical pair（impurities 1,2 and Rb1） were chosen as a criterion,the optimal modes of separation on R1,Rg1,Re and Rb1 have been obtained.Chromatographic optimization function（COF） values were used as objective functions,the results show that model 2 is better optimized.The optimized method was validated and applied for quan-titative analysis of four saponins of Sanqi Chinese Medicinal Granules.Duration of chromatographic run was 20 min.The results indicate that the method is accurate,feasible,and fast.

关 键 词：溶剂/流速双梯度 整体柱 高效液相色谱法 三七中药配方颗粒 

分 类 号：TQ461[化学工程—制药化工]