基于纳米材料修饰的高灵敏性L-苯丙氨酸印迹溶胶-凝胶传感器的研究  

Study on Nano-materials Modified Highly Sensitive L-phenylalaine Imprinted Sol-Gel Sensor

作　　者：罗丽娟[1] 张朝晖[1,2] 张明磊[1] 胡宇芳[1] 杨潇[1] 聂利华[2] 

机构地区：[1]吉首大学化学化工学院,湖南吉首416000 [2]湖南大学化学生物传感与计量国家重点实验室,湖南长沙410082

出　　处：《分析测试学报》2011年第5期473-480,共8页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(21005030);湖南省教育厅重点资助项目(10A099);湖南大学化学传感与计量国家重点实验室开放基金资助项目(2008007);湖南省林产化工重点实验室开放基金资助项目(JDZ2009003)

摘　　要：将氧化锌纳米膜(ZnO-NFs)、多壁碳纳米管(MWNTs)、纳米铜颗粒(Cu-NPs)和印迹溶胶-凝胶聚合物(MIP)依次修饰到碳电极(CE)表面,制备了一种对L-苯丙氨酸具有特异识别能力的印迹电化学传感器。采用电子扫描显微镜(SEM)对各修饰电极进行形貌表征;采用循环伏安法(CV)、示差脉冲伏安法(DPV)、安培时间(I-t)和线性扫描伏安法(LSV)对印迹传感器的电化学性能及最优检测条件进行了探讨。结果表明,当扫速为100 mV/s,工作电压为0.15 V,溶液pH值为5.5时,该印迹传感器对L-苯丙氨酸的浓度响应线性范围为5.0×10-8～2.0×10-5 mol/L,检出限为3.9×10-9 mol/L。该印迹传感器成功用于实际血清样品的分析,回收率为98%～101%。An imprinted electrochemical sensor with specific recognition ability for L-phenylalaine was synthesized with multi-layer modified zinc oxide nanofilms（ZnO-NFs）,multi-walled carbon nanotubes（MWNTs）,copper nanoparticles（Cu-NPs） and sol-gel molecularly imprinted polymer（MIP） on the surface of carbon electrode in succession.The morphologies of the modified sensors were investigated by scanning electron microscope（SEM）.The electrochemical performance and the optimal conditions for detection of L-phenylalaine were investigated by cyclic voltammetry（CV）,differential pulse voltammetry（DPV）,amperometric I-t curve and linear sweep voltammetry（LSV）.When the scanning rate was 100 mV/s,operating voltage was 0.15 V and pH was 5.5,the calibration curve was linear over the L-phenylalaine concentration range of 5.0×10-8-2.0×10-5 mol/L with a limit of detection of 3.9×10-9 mol/L.This sensor was successfully employed to detect L-phenylalaine in human serum sample with recoveries of 98%-101%.

关 键 词：印迹传感器 L-苯丙氨酸 氧化锌纳米膜 多壁碳纳米管 纳米铜颗粒 

分 类 号：O657.1[理学—分析化学] R977.4[理学—化学]