新疆几种裂腹鱼类系统发育关系探讨  

Molecular Phylogeny of Schizothoracinae Fishes in Xinjiang Based on Mitochondrial Cytochrome b Gene Sequences

作　　者：杨天燕[1] 孟玮[1] 海萨[1] 张人铭[1] 郭焱[1] 

机构地区：[1]新疆维吾尔自治区水产科学研究所,新疆乌鲁木齐830000

出　　处：《干旱区研究》2011年第3期555-561,共7页Arid Zone Research

基　　金：国家自然科学基金项目(31060356);新疆维吾尔自治区自然科学基金项目(2009211A30)

摘　　要：通过PCR扩增获得了分布于新疆的裂腹鱼亚科(Schizothoracinae)4属7种鱼类的线粒体细胞色素b基因712 bp序列。结果表明:Cyt b基因片段的T,C,A和G碱基组成平均为30.1%,27.4%,25.6%和16.9%,共出现201个变异位点,其中简约信息位点162个,序列变异度为22.8%,转换/颠换为15.03,且碱基替代主要发生在密码子第3位点。基于Kimura双参数模型构建NJ和MP系统树以及基于最佳核苷酸替代模型,构建ML系统树结果均显示,同为原始等级裂腹鱼类的扁吻鱼和裂腹鱼属鱼类亲缘关系较近,而特化等级裂腹鱼类的斑重唇鱼和新疆裸重唇鱼亲缘关系较近。依据分子进化速率推测两个类群间发生分歧的时间约在上新世晚期到中新世间,伴随青藏高原隆起这一地质事件。Totally 712 bp fragments of mitochondrial cytochrome b gene of 7 species in 4 genera of Schizothoracinae fishes in Xinjiang were amplified and sequenced.For the Cyt b gene,the average nucleotide contents of T,C,A and G were 30.1%,27.4%,25.6% and 16.9% respectively.There were 201 variable sites,of which 162 were parsimony-informative sites,their sequence variability was 22.8%,the transition/transversition was 15.03,and the nucleotide substitution occurred mostly at the third position.Three molecular phylogenetic trees were constructed with different methods,in which the neighbor-joining（NJ） tree and maximum parsimony（MP） tree were based on Kimura 2-parameter method,and the maximum likelihood（ML） tree was based on the best nucleotide substitution model.The results show that there were the close genetic relationships among the primordial-grade Schizothoracinae species and among the specialized-grade Schizothoracinae species.It is referred from the molecular evolution rate that the divergence of the 7 Schizothoracinae species occurred during the period from the late Pliocene to the Miocene with the uplift of the Qinghai-Tibet Plateau.

关 键 词：裂腹鱼类 线粒体 细胞色素B基因 系统发育 新疆 

分 类 号：S917.4[农业科学—水产科学]