玉米抗弯孢菌叶斑病的快速鉴定方法  

Rapid Evaluation Method of Corn Curvularia Leaf Spot Disease

作　　者：叶克苁 郭新梅[1] 魏志刚 张恩盈[1] 宋希云[1] 

机构地区：[1]青岛农业大学青岛市主要农作物种质资源创新与应用重点实验室,山东青岛266109 [2]青岛市种子站,山东青岛266071

出　　处：《西北农业学报》2011年第4期168-172,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：青岛市自然科学基金(05-2-JC-55);青岛市科技攻关项目(07-2-3-6-JCH);山东省泰山学者岗位

摘　　要：为了探寻一种室内初步鉴定玉米自交系弯孢菌叶斑病抗病性的方法,比较新月弯孢菌粗毒素与纱布过滤的菌液对玉米种子萌发的影响,分析种子生长抑制情况与田间抗病性的关系。结果表明,纱布过滤的菌液中蛋白质及多糖质量浓度高于粗毒素中两者的质量浓度,在稀释同等倍数的前提下,两者的致病力之间没有显著差异,且过滤的菌液对种子萌发、胚芽及胚根的生长抑制情况与田间抗病性的表现基本一致,可作为初步衡量玉米自交系弯孢菌叶斑病抗病性的方法。In order to find a primary evaluation method of maize for resistance to Curvularia leaf spot disease,the pathogenicity of crude toxin and filtrate was compared,and the relationship between seedling growth and inbred lines＇ resistance was analyzed in this paper.The results showed that the content of protein and polycose in filtrate was more than that in crude toxin.In the same dilution rate,the crude toxin and filtrate had no significant difference in pathogenicity.The filtrate inhibition to seeds germination and plantule and radicle growth was positive correlated with field resistance of inbred lines,so it could be used as a simple method to preliminary assess the resistance of inbred lines.

关 键 词：玉米 玉米弯孢菌叶斑病 新月弯孢菌 鉴定方法 粗毒素 滤液 

分 类 号：S435.131.49[农业科学—农业昆虫与害虫防治]